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Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium
- Source :
- REPRODUCTION. 145:453-462
- Publication Year :
- 2013
- Publisher :
- Bioscientifica, 2013.
-
Abstract
- This study has evaluated the performance of a multivariate statistical model to predict embryo implantation potential by processing data from the chemical fingerprinting of culture medium samples used for human embryo culture. The culture medium for 113 embryos from 55 patients undergoing ICSI was collected after embryo transfer. The samples were split into positive (n=29) and negative (n=84) implantation groups according their implantation outcomes (100% or 0% implantation). The samples were individually diluted and analyzed by electrospray ionization mass spectrometry (ESI-MS). Them/zratios and relative abundances of the major ions in each spectrum were considered for partial least square discriminant analysis. Data were divided into two subsets (calibration and validation), and the models were evaluated and applied to the validation set. A total of 5987 ions were observed in the groups. The multivariate statistical model described more than 82% of the data variability. Samples of the positive group were correctly identified with 100% probability and negative samples with 70%. The culture media used for embryos that were positive or negative for successful implantation showed specific biochemical signatures that could be detected in a fast, simple, and noninvasive way by ESI-MS. To our knowledge, this is the first report that uses MS fingerprinting to predict human embryo implantation potential. This biochemical profile could help the selection of the most viable embryo, improving single-embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies.
- Subjects :
- Adult
Fetal Proteins
Male
Spectrometry, Mass, Electrospray Ionization
Embryology
Calibration and validation
Data variability
Zygote
Electrospray ionization
Biology
Mass spectrometry
Models, Biological
Peptide Mapping
Endocrinology
Humans
Embryo Implantation
Sperm Injections, Intracytoplasmic
Infertility, Male
Chromatography
Discriminant Analysis
Obstetrics and Gynecology
Embryo
Cell Biology
Middle Aged
Embryo Transfer
Embryo transfer
Reproductive Medicine
Case-Control Studies
Culture Media, Conditioned
Calibration
Ectogenesis
Female
Multivariate statistical
Infertility, Female
Chemical fingerprinting
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 145
- Database :
- OpenAIRE
- Journal :
- REPRODUCTION
- Accession number :
- edsair.doi.dedup.....bbfa40fe2e60bd026e0c670a1acba267
- Full Text :
- https://doi.org/10.1530/rep-12-0168