Back to Search Start Over

Macrophage appearance in the epineurium and endoneurium of dorsal root ganglion exposed to nucleus pulposus

Authors :
Kjell Olmarker
Björn Rydevik
Kazuhisa Takahashi
Ichiro Takahashi
Yasuaki Murata
Source :
Journal of the Peripheral Nervous System. 9:158-164
Publication Year :
2004
Publisher :
Wiley, 2004.

Abstract

Recently, it was observed that local application of nucleus pulposus may induce a characteristic reaction at the surface of the dorsal root ganglion (DRG). This change was inflammatory in nature and occurred as early as 1 day after the application of nucleus pulposus. Herniation of the nucleus pulposus was surgically induced in the L4-5 disc in rats. The L4 DRGs were resected 3, 24, and 72 h after surgery and sectioned. The sections were processed for immunohistochemistry using antisera to the macrophage marker ED1 and observed using light microscopy. The appearance of macrophages was confirmed 3, 24, and 72 h after the surgery. Macrophages were mainly distributed in the epineurial space of the DRG 3 h after disc incision and also in the endoneurial tissue 24 and 72 h after disc incision. The immunoreactivity was significantly stronger at 24 and 72 h than at 3 h in the parts of the DRG without apparent changes in the disc incision series (p < 0.01). Within the epineurium adjacent to application of nucleus pulposus, the number was significantly higher at 3 h than at 24 and 72 h (p < 0.05). We conclude that experimental disc herniation with leakage of nucleus pulposus results in macrophage recruitment to the epineurium of the DRG 3 h after disc incision and to the endoneurium 24 and 72 h after disc incision.

Details

ISSN :
15298027 and 10859489
Volume :
9
Database :
OpenAIRE
Journal :
Journal of the Peripheral Nervous System
Accession number :
edsair.doi.dedup.....bbded4c234dbebbe1936f0425c383060
Full Text :
https://doi.org/10.1111/j.1085-9489.2004.09305.x