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Effect of intradermal injection of methionine-enkephalin on human skin
- Source :
- Nissen, JB, Egekvist, H, Bjerring, P & Kragballe, K 1999, ' Effect of intradermal injection of methionine-enkephalin on human skin ', Acta Dermato Venereologica, vol. 79, pp. 23-26 .
- Publication Year :
- 1999
-
Abstract
- Methionine-enkephalin (met-enk) detected in monocytes in psoriatic skin can modulate inflammatory processes and keratinocyte differentiation/proliferation in vitro. The purpose of the present study was to determine the effect of intradermal injection of met-enk on normal human skin and on the development of a delayed type skin hypersensitivity reaction. In 6 healthy volunteers, 50 microl of met-enk (16, 30, and 45 nmol) was injected once in the forearm and the reaction was evaluated clinically and by video-optical recording for 120 min. Compared to vehicle (0.9% saline), met-enk induced a time- and dose-dependent flare reaction, but no significant stimulation of a weal reaction. The flare reaction was maximal after 1 min and disappeared within 45 min. Pre-treatment with the antihistamine cetirizine reduced the flare reaction. Furthermore, the effect of met-enk on lymphocyte/monocyte infiltration and epidermal proliferation in normal skin and on a delayed type skin hypersensitivity reaction was assessed. Met-enk (45 nmol/ 50 microl) was injected at 0, 24 and 48 h. In normal skin, met-enk increased the number of dermal lymphocytes/monocytes (CD3/CD68 positive cells) and the degree of epidermal proliferation (MIBI-Ki67). In a delayed type hypersensitivity reaction induced by tuberculin (PPD), the degree of epidermal proliferation and the number of infiltrating lymphocytes/monocytes were reduced compared to PPD alone. Our study suggests that intradermal injection of met-enk in normal human skin induces an inflammatory reaction that may involve the release of histamine. In contrast, met-enk seems to down-regulate the development of a delayed type skin hypersensitivity reaction. These results may indicate that the direction of the effect of the opioid peptide met-enk on human skin depends on the rate of epidermal proliferation and the activity of immunocompetent cells.
- Subjects :
- Adult
Male
endocrine system
medicine.medical_specialty
Time Factors
CD3 Complex
Injections, Intradermal
Lymphocyte
Enkephalin, Methionine
Antigens, Differentiation, Myelomonocytic
Stimulation
Human skin
Dermatology
Tuberculin
Monocytes
chemistry.chemical_compound
Antigens, CD
Internal medicine
Anti-Allergic Agents
medicine
Humans
Intradermal injection
Lymphocytes
Skin
integumentary system
business.industry
CD68
Monocyte
General Medicine
Middle Aged
Immunohistochemistry
Cetirizine
Hypersensitivity reaction
medicine.anatomical_structure
Endocrinology
Ki-67 Antigen
chemistry
Erythema
Female
business
Histamine
Cell Division
Subjects
Details
- ISSN :
- 00015555
- Volume :
- 79
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Acta dermato-venereologica
- Accession number :
- edsair.doi.dedup.....bbc7dc4c8c0703b028630834ce442d6f