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The role of afferents to the ventral tegmental area in the handling stress-induced increase in the release of dopamine in the medial prefrontal cortex: a dual-probe microdialysis study in the rat brain
- Source :
- Brain Research, 779(1-2), 205-213. ELSEVIER SCIENCE BV, Brain Research, 205-213. ELSEVIER SCIENCE BV, STARTPAGE=205;ENDPAGE=213;ISSN=0006-8993;TITLE=Brain Research
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- This study was aimed to identify the neuronal pathways that mediate the handling stress induced increase in the release of dopamine in the medial prefrontal cortex (mPFC) of the rat brain. For that purpose a microdialysis probe was implanted in the ventral tegmental area (VTA) and a second probe was placed in the ipsilateral mPFC. Receptor specific compounds acting on GABA(A) (20 mu M muscimol), GABA(B) (50 mu M baclofen), acetylcholine (100 mu M atropine, 100 mu M mecamylamine), NMDA (30, 100 and 300 mu M CPP; 300 mu M AP-5, 1 mM (+)-HA-966) and non-NMDA receptors (500 mu M CNQX) were infused into the VTA by retrograde dialysis, whereas extracellular dopamine was recorded in the ipsilateral mPFC. Intrategmental infusion of muscimol, baclofen, CPP, AP-5, (+)-HA-966 and CNQX decreased extracellular dopamine in the ipsilateral mPFC; atropine and mecamylamine were without effect on the basal values. During infusion of the various compounds rats were gently handled for 15 min. The infusions of muscimol, atropine, mecamylamine and(+)-HA-966 did not modify the handling stress induced increase in extracellular dopamine in the mPFC. However, during intrategmental infusion of baclofen, CPP, AP-5 and CNQX the handling stress induced increase in extracellular dopamine (expressed as % of controls) in the mPFC was suppressed. These results indicate that a glutamatergic projection to the VTA, acting via both NMDA and non-NMDA-glutamate receptors, play a major role in the handling stress-induced increase in dopamine release in the mPFC. In addition the results suggest a certain role for GABAergic neurones, acting via GABA(B) receptors, in the handling response. (C) 1998 Elsevier Science B.V.
- Subjects :
- Male
Baclofen
Dopamine
Microdialysis
Mecamylamine
stress
chemistry.chemical_compound
Receptors, Cholinergic
NEURONS
IN-VIVO
Muscimol
CORTICAL DOPAMINE
musculoskeletal, neural, and ocular physiology
General Neuroscience
FRONTAL-CORTEX
Ventral tegmental area
medicine.anatomical_structure
Receptors, Glutamate
CNQX
DIAZEPAM
METHYL-D-ASPARTATE
VTA
psychological phenomena and processes
MESOCORTICOLIMBIC DOPAMINE
medicine.drug
medicine.medical_specialty
NUCLEUS-ACCUMBENS
Prefrontal Cortex
GABAB receptor
Nucleus accumbens
Handling, Psychological
Receptors, GABA
Stress, Physiological
Internal medicine
EXCITATORY PATHWAY
medicine
Animals
Neurons, Afferent
Rats, Wistar
Molecular Biology
RECEPTOR
Ventral Tegmental Area
Rats
Endocrinology
NMDA
nervous system
chemistry
Neurology (clinical)
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 779
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....bb97bddbd981094f2504cd0deb4563a9