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Supramolecular modification of a sequence-controlled collagen-mimicking polymer
- Source :
- Biomacromolecules, 20(6), 2360-2371. American Chemical Society
- Publication Year :
- 2019
-
Abstract
- [Image: see text] Structurally and functionally well-defined recombinant proteins are an interesting class of sequence-controlled macromolecules to which different crosslinking chemistries can be applied to tune their biological properties. Herein, we take advantage of a 571-residue recombinant peptide based on human collagen type I (RCPhC1), which we functionalized with supramolecular 4-fold hydrogen bonding ureido-pyrimidinone (UPy) moieties. By grafting supramolecular UPy moieties onto the backbone of RCPhC1 (UPy-RCPhC1), increased control over the polymer structure, assembly, gelation, and mechanical properties was achieved. In addition, by increasing the degree of UPy functionalization on RCPhC1, cardiomyocyte progenitor cells were cultured on “soft” (∼26 kPa) versus “stiff” (∼68–190 kPa) UPy-RCPhC1 hydrogels. Interestingly, increased stress fiber formation, focal adhesions, and proliferation were observed on stiffer compared to softer substrates, owing to the formation of stronger cell–material interactions. In conclusion, a bioinspired hydrogel material was designed by a combination of two well-known natural components, i.e., a protein as sequence-controlled polymer and UPy units inspired on nucleobases.
- Subjects :
- Polymers and Plastics
Supramolecular chemistry
Bioengineering
Sequence (biology)
02 engineering and technology
010402 general chemistry
01 natural sciences
Collagen Type I
Biomaterials
Focal adhesion
Mice
Biomimetic Materials
Materials Chemistry
Animals
Humans
Myocytes, Cardiac
Cell Line, Transformed
Cell Proliferation
chemistry.chemical_classification
Chemistry
Hydrogen bond
Stem Cells
Polymer
021001 nanoscience & nanotechnology
0104 chemical sciences
Self-healing hydrogels
Biophysics
Surface modification
0210 nano-technology
Macromolecule
Subjects
Details
- Language :
- English
- ISSN :
- 15257797
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules, 20(6), 2360-2371. American Chemical Society
- Accession number :
- edsair.doi.dedup.....bb92cba652bef361995537ce4089c727