Back to Search
Start Over
Uncovering Intense Protein Diversification in a Cone Snail Venom Gland Using an Integrative Venomics Approach
Uncovering Intense Protein Diversification in a Cone Snail Venom Gland Using an Integrative Venomics Approach
- Source :
- Journal of Proteome Research. 14:628-638
- Publication Year :
- 2015
- Publisher :
- American Chemical Society (ACS), 2015.
-
Abstract
- Marine cone snail venoms are highly complex mixtures of peptides and proteins. They have been studied in-depth over the past 3 decades, but the modus operandi of the venomous apparatus still remains unclear. Using the fish-hunting Conus consors as a model, we present an integrative venomics approach, based on new proteomic results from the venom gland and data previously obtained from the transcriptome and the injectable venom. We describe here the complete peptide content of the dissected venom by the identification of numerous new peptides using nanospray tandem mass spectrometry in combination with transcriptomic data. Results reveal extensive mature peptide diversification mechanisms at work in the venom gland. In addition, by integrating data from three different venom stages, transcriptome, dissected, and injectable venoms, from a single species, we obtain a global overview of the venom processing that occurs from the venom gland tissue to the venom delivery step. In the light of the successive steps in this venom production system, we demonstrate that each venom compartment is highly specific in terms of peptide and protein content. Moreover, the integrated investigative approach discussed here could become an essential part of pharmaceutical development, as it provides new potential drug candidates and opens the door to numerous analogues generated by the very mechanisms used by nature to diversify its peptide and protein arsenal.
- Subjects :
- Proteomics
Molecular Sequence Data
Venom
Peptide
Computational biology
Biology
Bioinformatics
complex mixtures
Biochemistry
Mass Spectrometry
Cone snail
Protein content
Transcriptome
Single species
Animals
Amino Acid Sequence
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Sequence Homology, Amino Acid
Conus Snail
General Chemistry
Venom gland
biology.organism_classification
chemistry
Conus consors
Conotoxins
Subjects
Details
- ISSN :
- 15353907 and 15353893
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Proteome Research
- Accession number :
- edsair.doi.dedup.....bb6d6ae6a6bc13959a172f5205a5c0d4
- Full Text :
- https://doi.org/10.1021/pr500583u