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New packaging method of mycobacterial cell wall using octaarginine-modified liposomes: Enhanced uptake by and immunostimulatory activity of dendritic cells
- Source :
- Journal of Controlled Release. 120:60-69
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Despite the potential of mycobacterial cell wall (CW) components to serve as immunotherapeutic agents, this application is hampered by the molecules' unfavorable physicochemical properties, such as its high molecular weight, poor solubility and negatively charged nature. Here we describe a new mycobacterial CW delivery system that uses an efficient and simple packaging method. This is achieved by incorporating mycobacterial CW into liposomes and attaching arginine octamers (R8) to the liposome surface. R8-modified liposomes improve the uptake of mycobacterial CW by dendritic cells (DC) and enhance its immunostimulatory activity. High R8 surface density promoted high levels of mycobacterial CW uptake by DC compared to low density R8-modified liposomes. Maturation markers (CD80, CD86, MHC Class II molecules) showed significantly enhanced expression on DC pulsed with high density R8-modified liposomes containing mycobacterial CW. Moreover, R8-modified liposomes with mycobacterial CW incorporated induced production of IL-12 p40 by DC, at levels similar to those produced by lipopolysaccharide-pulsed DC. We assert that R8-modified liposomes with mycobacterial CW incorporated should have tremendous potential as immune-potentiating agents.
- Subjects :
- Male
Arginine
medicine.drug_class
Chemistry, Pharmaceutical
Drug Compounding
Pharmaceutical Science
Cancer Vaccines
Immunostimulant
Cell wall
Mice
Cell Wall
medicine
Animals
Particle Size
Cells, Cultured
CD86
MHC class II
Liposome
biology
Interleukin-12 Subunit p40
Histocompatibility Antigens Class II
Dendritic Cells
Dendritic cell
Lipids
Mycobacterium bovis
Mice, Inbred C57BL
Cholesterol
Solubility
Biochemistry
Liposomes
B7-1 Antigen
Phosphatidylcholines
biology.protein
Biophysics
B7-2 Antigen
Oligopeptides
CD80
Subjects
Details
- ISSN :
- 01683659
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Journal of Controlled Release
- Accession number :
- edsair.doi.dedup.....bb5366e12dac01ff58ecdba886b0ad34