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Adaptive Mutations in RNA Polymerase and the Transcriptional Terminator Rho Have Similar Effects on Escherichia coli Gene Expression
- Source :
- Molecular Biology and Evolution, González-González, Andrea; Hug, Shaun M; Rodríguez-Verdugo, Alejandra; Patel, Jagdish Suresh; & Gaut, Brandon S. (2017). Adaptive Mutations in RNA Polymerase and the Transcriptional Terminator Rho Have Similar Effects on Escherichia coli Gene Expression.. Molecular biology and evolution, 34(11), 2839-2855. doi: 10.1093/molbev/msx216. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/40j8s0dm, Molecular Biology and Evolution, 34 (11)
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Modifications to transcriptional regulators play a major role in adaptation. Here, we compared the effects of multiple beneficial mutations within and between Escherichia coli rpoB, the gene encoding the RNA polymerase β subunit, and rho, which encodes a transcriptional terminator. These two genes have harbored adaptive mutations in numerous E. coli evolution experiments but particularly in our previous large-scale thermal stress experiment, where the two genes characterized alternative adaptive pathways. To compare the effects of beneficial mutations, we engineered four advantageous mutations into each of the two genes and measured their effects on fitness, growth, gene expression and transcriptional termination at 42.2 °C. Among the eight mutations, two rho mutations had no detectable effect on relative fitness, suggesting they were beneficial only in the context of epistatic interactions. The remaining six mutations had an average relative fitness benefit of ∼20%. The rpoB mutations affected the expression of ∼1,700 genes; rho mutations affected the expression of fewer genes but most (83%) were a subset of those altered by rpoB mutants. Across the eight mutants, relative fitness correlated with the degree to which a mutation restored gene expression back to the unstressed, 37.0 °C state. The beneficial mutations in the two genes did not have identical effects on fitness, growth or gene expression, but they caused parallel phenotypic effects on gene expression and genome-wide transcriptional termination.<br />Molecular Biology and Evolution, 34 (11)<br />ISSN:0737-4038<br />ISSN:1537-1719
- Subjects :
- 0301 basic medicine
relative fitness
Mutant
adaptive pathways
Biology
Evolution, Molecular
phenotypic convergence
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
RNA polymerase
Gene expression
Escherichia coli
Genetics
experimental evolution
protein structure
Molecular Biology
Gene
Discoveries
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Terminator Regions, Genetic
Evolutionary Biology
0303 health sciences
transcriptional termination
Escherichia coli Proteins
DNA-Directed RNA Polymerases
Gene Expression Regulation, Bacterial
rpoB
Adaptation, Physiological
Phenotype
030104 developmental biology
Terminator (genetics)
chemistry
Mutation
Epistasis
Biochemistry and Cell Biology
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15371719 and 07374038
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Molecular Biology and Evolution
- Accession number :
- edsair.doi.dedup.....baebdff63d6f9e6c29feaed5d25ee3c7