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Catalase in manipulation buffer enhances the developmental competence of DNA-injected embryos
- Source :
- The Journal of reproduction and development. 50(6)
- Publication Year :
- 2005
-
Abstract
- To improve the efficiency of transgenesis, we investigated the effects of a radical scavenger during microinjection on the development to blastocysts or pups of mouse pronuclear embryos, microinjected with the enhanced green fluorescent protein (EGFP) transgene. When embryos were microinjected in medium containing 0-1,000 units/ml catalase, the developmental rate to blastocysts was significantly higher (P
- Subjects :
- Male
Genotype
Ontogeny
Transgene
Green Fluorescent Proteins
Green fluorescent protein
Embryo Culture Techniques
Mice
Animals
Transgenes
Microinjection
chemistry.chemical_classification
Cell Nucleus
Reactive oxygen species
biology
Gene Transfer Techniques
Embryo
DNA
Catalase
Embryo, Mammalian
Molecular biology
Spermatozoa
Transgenesis
Mice, Inbred C57BL
Blastocyst
chemistry
Genetic Techniques
embryonic structures
biology.protein
Animal Science and Zoology
Female
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 09168818
- Volume :
- 50
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Journal of reproduction and development
- Accession number :
- edsair.doi.dedup.....baeae7c3b9dbcf51213312f5d2e0dd29