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A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana

Authors :
Kazusato Oikawa
Keiko Midorikawa
Yutaka Kodama
Keiji Numata
Source :
Frontiers in Plant Science. 13
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

Organelles contribute to plant growth via their movements and interactions, which ensure efficient metabolic flow and help plants adapt to environmental stress. Live-cell imaging of the interactions of organelles has been performed in yeast, plant, and animal cells. However, high-throughput quantitative methods are needed to simultaneously analyze the interactions of many organelles in living plant cells. Here, we developed a semi-automatic high-throughput method to quantitatively evaluate the interactions between peroxisomes and chloroplasts using a distance transformation algorithm and high-resolution 3D fluorescent images taken by confocal laser scanning microscopy. Using this method, we measured the 3D distance between the center of peroxisome and chloroplast surface in Arabidopsis thaliana. We then compared the distances between these organelles in leaf mesophyll cells under light and dark conditions. This distance was shorter in the light than in the dark, which is in agreement with the findings of previous studies. We used our method to evaluate peroxisome–chloroplast (plastid) interactions in different cell types in the light and dark, including guard, stem, and root cells. Like in mesophyll cells, the distance between the peroxisome and chloroplast was shorter in the light in guard and stem cells, but not in root cells, suggesting that photosynthetic plastids (chloroplasts) play important roles in these interactions. When leaf mesophyll cells were incubated under high-intensity light, the frequency of shorter distances between peroxisomes and chloroplasts significantly increased. Our high-throughput, semi-automatic method represents a powerful tool for evaluating peroxisome–chloroplast interactions in different types of plant cells under various environmental conditions.

Subjects

Subjects :
Plant Science

Details

Language :
English
ISSN :
1664462X
Volume :
13
Database :
OpenAIRE
Journal :
Frontiers in Plant Science
Accession number :
edsair.doi.dedup.....badc8a81f8264538221cc0e98a634238
Full Text :
https://doi.org/10.3389/fpls.2022.998960