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Long non-coding RNA expression profile in Cdk5-knockdown mouse skin
- Source :
- Gene. 672:195-201
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- To elucidate the Cdk5 regulatory molecular mechanism in skin, we generated Cdk5-knockdown mice and subjected their skins to lncRNA sequencing. The results showed that there were 4533 novel lncRNAs from 142 lncRNA families. In total, 693 lncRNAs were significantly differentially expressed. Alignment analysis of the lncRNAs in miRBase identified 45 pre-mRNAs. By KEGG PATHWAY Database analysis, we found that lncRNAs (lnc-NONMMUT064276.2, lnc-NONMMUT075728.1, and lnc-NONMMUT039653.2) may regulate pigmentation by regulating target genes. To reveal potential antisense lncRNA-mRNA interactions, we searched all lncRNA-mRNA duplexes using RNAplex, and found 97 lncRNAs interacted with mRNAs. The luciferase assay confirmed that TCONS_00049140 binded to Krt80 by the co-transfection of pVAX1-TCONS_00049140 and pGL0-Krt80 expression plasmids in 293T cell, based on the bioinformatics analysis. Overexpression of TCONS_00049140 in mouse melanocytes down-regulated Krt80 and resulted in the phenotype of increased cell proliferation and increased melanin production. The results suggested that TCONS_00049140 contributed to skin thickening through Krt80. Our findings provide a direction for research of the molecular mechanism of Cdk5 function.
- Subjects :
- Male
0301 basic medicine
Biology
MiRBase
Mice
03 medical and health sciences
Genetics
Animals
Humans
Luciferase
Gene
Skin
Gene knockdown
Base Sequence
Cell growth
Cyclin-Dependent Kinase 5
General Medicine
Phenotype
Long non-coding RNA
Cell biology
HEK293 Cells
030104 developmental biology
Gene Knockdown Techniques
Melanocytes
RNA, Long Noncoding
Transcriptome
Function (biology)
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 672
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....bad4a8ba27a0e0b432a06ce9afaa5fcb