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Expression of insulin-like growth factors and their binding proteins by bronchoalveolar cells from children with and without interstitial lung disease
- Source :
- European Respiratory Journal. 11:1329-1336
- Publication Year :
- 1998
- Publisher :
- European Respiratory Society (ERS), 1998.
-
Abstract
- The involvement of the insulin-like growth factor (IGF) system in lung growth and repair following injury is sustained by a number of studies. Based on this knowledge, the aim of the present work was to document the expression of the IGFs and their binding proteins by alveolar cells obtained by bronchoalveolar lavage (BAL). Two groups were investigated: a control group of five children and a group of 11 children referred to the department for exploration of interstitial lung disease (ILD). Components of the IGF system studied included IGF-I, IGF-II and IGF-binding proteins (IGFBP). Expression of these factors was analysed at the level of messenger ribonucleic acid (mRNA) (by semi-quantitative reverse transcription polymerase chain reaction techniques), and of protein for the IGFBPs. In addition, expression of two major cytokines associated with the inflammatory process, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta), was also documented. In children without parenchymal disease, the growth factor expressed was IGF-I, in association with the presence of mRNA for IGFBP-2 in all cases. In children with ILD, expression of IGF-I was observed in nine patients and of IGF-II in three patients, and the presence of IGFBP-2 was found in all extracts analysed (mRNA and proteins). Evaluation of IGFBP-2 expression indicated an increase in the group of children with ILD. Interestingly, a significant association was observed between the increase in IGFBP-2 expression and TGF-beta expression. The present data emphasize the presence on insulin-like growth factor-binding protein-2 in the BAL of all patients, and suggest that this protein may be an important factor of the injury/repair processes during the progression of alveolar inflammation.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Adolescent
medicine.medical_treatment
Cell Count
Inflammation
Polymerase Chain Reaction
Alveolar cells
Somatomedins
Transforming Growth Factor beta
Internal medicine
medicine
Humans
RNA, Messenger
Insulin-Like Growth Factor I
Child
Messenger RNA
Lung
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
business.industry
Growth factor
Interstitial lung disease
medicine.disease
Insulin-Like Growth Factor Binding Proteins
Pulmonary Alveoli
Reverse transcription polymerase chain reaction
Insulin-Like Growth Factor Binding Protein 2
Bronchoalveolar lavage
medicine.anatomical_structure
Endocrinology
Child, Preschool
Female
medicine.symptom
Lung Diseases, Interstitial
business
Bronchoalveolar Lavage Fluid
Subjects
Details
- ISSN :
- 13993003 and 09031936
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- European Respiratory Journal
- Accession number :
- edsair.doi.dedup.....bad187b281fbbce339638697807f3ea3