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Circular RNA CDR1as Inhibits the Metastasis of Gastric Cancer through Targeting miR-876-5p/GNG7 Axis
- Source :
- Gastroenterology Research and Practice, Vol 2021 (2021), Gastroenterology Research and Practice
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Circular RNA CDR1as has been demonstrated to participate in various cancer progressions as miRNA sponges. The exact underlying mechanisms of CDR1as on gastric cancer (GC) metastasis remain unknown. Here, we found that CDR1as knockdown facilitated GC cell migration and invasion while its overexpression inhibited the migration and invasion abilities of GC cells in vitro and in vivo. Moreover, epithelial-mesenchymal transition- (EMT-) associated proteins and MMP2 and MMP9 were downregulated by CDR1as. Bioinformatics analysis combined with dual-luciferase reporter gene assays, western blot, RT-qPCR analysis, and functional rescue experiments demonstrated that CDR1as served as a miR-876-5p sponge and upregulated the target gene GNG7 expression to suppress GC metastasis. In summary, our findings indicate that CDR1as suppresses GC metastasis through the CDR1as/miR-876-5p/GNG7 axis.
- Subjects :
- 0301 basic medicine
Reporter gene
Gene knockdown
MMP2
Hepatology
Article Subject
business.industry
Gastroenterology
Cancer
Cell migration
RC799-869
Diseases of the digestive system. Gastroenterology
medicine.disease
Metastasis
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Circular RNA
030220 oncology & carcinogenesis
microRNA
Cancer research
Medicine
business
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 16876121
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Gastroenterology Research and Practice
- Accession number :
- edsair.doi.dedup.....ba9eb2def6eeb4cb10e001cecb3f850a