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Abscopal effects of radiotherapy and combined mRNA-based immunotherapy in a syngeneic, OVA-expressing thymoma mouse model
- Source :
- Cancer immunology, immunotherapy : CII. 67(4)
- Publication Year :
- 2017
-
Abstract
- Tumor metastasis and immune evasion present major challenges of cancer treatment. Radiotherapy can overcome immunosuppressive tumor microenvironments. Anecdotal reports suggest abscopal anti-tumor immune responses. This study assesses abscopal effects of radiotherapy in combination with mRNA-based cancer vaccination (RNActive®). C57BL/6 mice were injected with ovalbumin-expressing thymoma cells into the right hind leg (primary tumor) and left flank (secondary tumor) with a delay of 4 days. Primary tumors were irradiated with 3 × 2 Gy, while secondary tumors were shielded. RNA and combined treatment groups received mRNA-based RNActive® vaccination. Radiotherapy and combined radioimmunotherapy significantly delayed primary tumor growth with a tumor control in 15 and 53% of mice, respectively. In small secondary tumors, radioimmunotherapy significantly slowed growth rate compared to vaccination (p = 0.002) and control groups (p = 0.01). Cytokine microarray analysis of secondary tumors showed changes in the cytokine microenvironment, even in the non-irradiated contralateral tumors after combination treatment. Combined irradiation and immunotherapy is able to induce abscopal responses, even with low, normofractionated radiation doses. Thus, the combination of mRNA-based vaccination with irradiation might be an effective regimen to induce systemic anti-tumor immunity.
- Subjects :
- 0301 basic medicine
Cancer Research
Thymoma
Ovalbumin
medicine.medical_treatment
Immunology
Metastasis
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Immunology and Allergy
Animals
RNA, Messenger
Tumor microenvironment
business.industry
Cancer
Antibodies, Monoclonal
Immunotherapy
Thymus Neoplasms
Radioimmunotherapy
medicine.disease
Primary tumor
Combined Modality Therapy
Radiation therapy
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
business
Subjects
Details
- ISSN :
- 14320851
- Volume :
- 67
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cancer immunology, immunotherapy : CII
- Accession number :
- edsair.doi.dedup.....ba99453c1588932379beb47c5f69358a