Salmonella Infantis and Salmonella Enteritidis specific bacteriophages isolated form poultry faeces as a complementary tool for cleaning and disinfection against Salmonella

Este artículo se encuentra disponible en la siguiente URL: https://www.sciencedirect.com/science/article/abs/pii/S0147957119302024?via%3Dihub This is a pre-print of an article published in Sevilla-Navarro, S., Catalá-Gregori, P., García, C., Cortés, V., & Marin, C. (2020). Salmonella Infantis and Salmonella Enteritidis specific bacteriophages isolated form poultry faeces as a complementary tool for cleaning and disinfection against Salmonella. Comparative immunology, microbiology and infectious diseases, vol. 68, art. 101405. The final authenticated version is available online at: https://doi.org/10.1016/j.cimid.2019.101405 Este es el pre-print del siguiente artículo: Sevilla-Navarro, S., Catalá-Gregori, P., García, C., Cortés, V., & Marin, C. (2020). Salmonella Infantis and Salmonella Enteritidis specific bacteriophages isolated form poultry faeces as a complementary tool for cleaning and disinfection against Salmonella. Comparative immunology, microbiology and infectious diseases, vol. 68, art. 101405, que se ha publicado de forma definitiva en https://doi.org/10.1016/j.cimid.2019.101405 Salmonellosis represents an important public health concern. Several authors point out the inefficiency of the cleaning and disinfection protocols to remove the bacteria from the field. For this reason, innovative techniques, as bacteriophages, could be implemented to control the bacteria. The main objectives of this study were to assess the effect of bacteriophages against Salmonella Infantis and Salmonella Enteritidis on farm surfaces, and to evaluate bacteriophage procedure application as sanitiser against Salmonella in field conditions. Thus, most prevalent serovars in poultry production were selected (Salmonella Infantis and Salmonella Enteritidis) to contaminate farm facilities. Then, two specific bacteriophages isolated from poultry faeces were applied against them. Results showed Salmonella Infantis and Salmonella Enteritidis decreased of 4.55 log10CFU/mL and 3.85 log10CFU/mL, respectively; the maximum reduction in Salmonella was the 5th day, after 108 PFU/mL and 103 PFU/mL bacteriophage application. These results highlight bacteriophages as a promising tool together with cleaning and disinfection. Preprint