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A dual center study to compare breath volatile organic compounds from smokers and non-smokers with and without COPD
- Source :
- Journal of Breath Research 10 (2016), Nr. 2
- Publication Year :
- 2016
- Publisher :
- Bristol : Institute of Physics Publishing, 2016.
-
Abstract
- There is increasing evidence that breath volatile organic compounds (VOC) have the potential to support the diagnosis and management of inflammatory diseases such as COPD. In this study we used a novel breath sampling device to search for COPD related VOCs. We included a large number of healthy controls and patients with mild to moderate COPD, recruited subjects at two different sites and carefully controlled for smoking. 222 subjects were recruited in Hannover and Marburg, and inhaled cleaned room air before exhaling into a stainless steel reservoir under exhalation flow control. Breath samples (2.5 l) were continuously drawn onto two Tenax(®) TA adsorption tubes and analyzed in Hannover using thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). Data of 134 identified VOCs from 190 subjects (52 healthy non-smokers, 52 COPD ex-smokers, 49 healthy smokers, 37 smokers with COPD) were included into the analysis. Active smokers could be clearly discriminated by higher values for combustion products and smoking related VOCs correlated with exhaled carbon monoxide (CO), indicating the validity of our data. Subjects from the study sites could be discriminated even after exclusion of cleaning related VOCs. Linear discriminant analysis correctly classified 89.4% of COPD patients in the non/ex-smoking group (cross validation (CV): 85.6%), and 82.6% of COPD patients in the actively smoking group (CV: 77.9%). We extensively characterized 134 breath VOCs and provide evidence for 14 COPD related VOCs of which 10 have not been reported before. Our results show that, for the utilization of breath VOCs for diagnosis and disease management of COPD, not only the known effects of smoking but also site specific differences need to be considered. We detected novel COPD related breath VOCs that now need to be tested in longitudinal studies for reproducibility, response to treatment and changes in disease severity.
- Subjects :
- Adult
Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Pathology
Copd patients
01 natural sciences
Gas Chromatography-Mass Spectrometry
Pulmonary Disease, Chronic Obstructive
Young Adult
03 medical and health sciences
0302 clinical medicine
Disease severity
Internal medicine
medicine
Humans
ddc:610
Aged
Volatile Organic Compounds
COPD
Non-invasive monitoring
business.industry
Smoking
010401 analytical chemistry
Breath sampling
Reproducibility of Results
Exhalation
Breath analysis
Exhaled breath
Middle Aged
medicine.disease
Response to treatment
Body Fluids
0104 chemical sciences
respiratory tract diseases
Breath Tests
030228 respiratory system
Breath gas analysis
Combustion products
Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit
business
Airway inflammation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Breath Research 10 (2016), Nr. 2
- Accession number :
- edsair.doi.dedup.....ba6a79edb929de64bef9169cc3863574