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ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome
- Source :
- Canadian Respiratory Journal, Canadian Respiratory Journal, Vol 2018 (2018)
- Publication Year :
- 2018
- Publisher :
- Hindawi, 2018.
-
Abstract
- Background. Hemeoxygenase-1 (HO-1) is an essential enzyme in heme catabolism and has been proposed as a biomarker of lung disease prognosis. We modified a commercial HO-1 enzyme-linked immunosorbent assay (ELISA) kit to achieve higher sensitivity and evaluated if serum HO-1 could be a biomarker to predict the prognosis of acute respiratory distress syndrome (ARDS) patients.Methods. Serum samples were collected from 15 healthy volunteers to validate the modified ELISA. In the 22 patients with ARDS who were enrolled, serum HO-1 was measured upon diagnosis (D0) and at 7 days after diagnosis (D7).Results. The serum HO-1 concentration could be measured in all healthy volunteers. The intra- and interassay tests and the percentage recovery test were acceptable. Compared with normal control subjects, patients with ARDS had significantly higher D0 HO-1 concentrations (75.4 ng/mL versus 31.7 ng/mL,P<0.001). The 28-day survival was significantly better in patients with low D0 HO-1 (P=0.016). Nonsurvivors had significantly higher D0 and D7 HO-1 concentrations than survivors (P<0.05).Conclusion. Serum HO-1 may be a useful biomarker to predict the prognosis of patients with ARDS.
- Subjects :
- Pulmonary and Respiratory Medicine
Adult
Male
medicine.medical_specialty
ARDS
Article Subject
Enzyme-Linked Immunosorbent Assay
Acute respiratory distress
030204 cardiovascular system & hematology
Gastroenterology
Sensitivity and Specificity
Hemeoxygenase 1
Diseases of the respiratory system
03 medical and health sciences
0302 clinical medicine
Internal medicine
Medicine
Humans
In patient
Survival rate
Aged
Respiratory Distress Syndrome
RC705-779
business.industry
Case-control study
Middle Aged
medicine.disease
Prognosis
Survival Rate
030228 respiratory system
Lung disease
Case-Control Studies
Biomarker (medicine)
Female
business
Heme Oxygenase-1
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 11982241
- Database :
- OpenAIRE
- Journal :
- Canadian Respiratory Journal
- Accession number :
- edsair.doi.dedup.....ba6893050123fa67aa4167a4f2811771
- Full Text :
- https://doi.org/10.1155/2018/9627420