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Cloning and expression of the B chain of volkensin, type 2 ribosome inactivating protein from Adenia volkensii harms: co-folding with the A chain for heterodimer reconstitution
- Source :
- Protein expression and purification. 51(2)
- Publication Year :
- 2006
-
Abstract
- Type 2 ribosome inactivating proteins (RIPs) include some potent plant toxins, among which ricin from Ricinus communis and abrin from Abrus precatorius seeds, have been known for more than a century. Two other type 2 RIPs belong to this class of proteins, both isolated from plants of the same family (Passifloraceae), modeccin and volkensin, from Adenia digitata and Adenia volkensii roots, respectively. Volkensin is probably the most potent plant toxin known, with an LD50 for rats of 50-60 ng/kg. Here we report the cloning, expression and renaturation of recombinant volkensin B chain. Furthermore, starting from separately expressed A and B chains, a co-association procedure was set-up, leading to in vitro heterodimeric volkensin reconstitution. The recombinant heterodimer was characterized by N-terminal sequence analysis and its hemagglutinating activity assessed. In parallel, we have explored the carbohydrate-binding properties of native volkensin with the aim to correlate toxin-specific properties (i.e., axonal transport along neurons) to lectin's sugar-binding preferences. © 2006 Elsevier Inc. All rights reserved.
- Subjects :
- Protein Folding
Volkensin
Enzyme-Linked Immunosorbent Assay
Chromatography, Affinity
law.invention
chemistry.chemical_compound
law
Abrus precatorius
Humans
Refolding
Cloning, Molecular
N-Glycosyl Hydrolases
Glycoproteins
Cloning
biology
Ribosome-inactivating protein
Ricinus
Lectin
Hemagglutination Tests
Plant lectin
biology.organism_classification
Molecular biology
Recombinant Proteins
Ribosome Inactivating Proteins, Type 2
Ricin
chemistry
Biochemistry
Recombinant DNA
biology.protein
Electrophoresis, Polyacrylamide Gel
Abrin
Plant Lectins
Dimerization
Biotechnology
Subjects
Details
- ISSN :
- 10465928
- Volume :
- 51
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Protein expression and purification
- Accession number :
- edsair.doi.dedup.....ba5e6a1c67dcac7f2e1fdbfe98282ff2