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Metatranscriptomics of Soil Eukaryotic Communities
- Source :
- Microbial Environmental Genomics (MEG), Francis Martin; Stephane Uroz. Microbial Environmental Genomics (MEG), 1399, HUMANA PRESS INC, pp.273-287, 2016, Methods in Molecular Biology, 978-1-4939-3369-3. ⟨10.1007/978-1-4939-3369-3_16⟩, Microbial Environmental Genomics (MEG) ISBN: 9781493933679, The Ecological Genomics of Fungi, Francis Martin; Stephane Uroz. Microbial Environmental Genomics (MEG), pp.273-287, 2016, 978-1-4939-3369-3. ⟨10.1007/978-1-4939-3369-3_16⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Functions expressed by eukaryotic organisms in soil can be specifically studied by analyzing the pool of eukaryotic-specific polyadenylated mRNA directly extracted from environmental samples. In this chapter, we describe two alternative protocols for the extraction of high-quality RNA from soil samples. Total soil RNA or mRNA can be converted to cDNA for direct high-throughput sequencing. Polyadenylated mRNA-derived full-length cDNA scan also be cloned in expression plasmid vectors to constitute soil cDNA libraries, which can be subsequently screened for functional gene categories. Alternatively, the diversity of specific gene families can also be explored following cDNA sequence capture using exploratory oligonucleotide probes.
- Subjects :
- 0301 basic medicine
cDNA libraries
Messenger RNA
Expression vector
cDNA library
Oligonucleotide
[SDV]Life Sciences [q-bio]
fungi
030106 microbiology
RNA
food and beverages
cDNA synthesis
Computational biology
Biology
Microbiology
03 medical and health sciences
030104 developmental biology
Metagenomics
Complementary DNA
Genomic library
cDNA size fractionation
ComputingMilieux_MISCELLANEOUS
Metatranscriptomics
Environmental RNA
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-3369-3
978-1-4939-3367-9 - ISBNs :
- 9781493933693 and 9781493933679
- Database :
- OpenAIRE
- Journal :
- Microbial Environmental Genomics (MEG), Francis Martin; Stephane Uroz. Microbial Environmental Genomics (MEG), 1399, HUMANA PRESS INC, pp.273-287, 2016, Methods in Molecular Biology, 978-1-4939-3369-3. ⟨10.1007/978-1-4939-3369-3_16⟩, Microbial Environmental Genomics (MEG) ISBN: 9781493933679, The Ecological Genomics of Fungi, Francis Martin; Stephane Uroz. Microbial Environmental Genomics (MEG), pp.273-287, 2016, 978-1-4939-3369-3. ⟨10.1007/978-1-4939-3369-3_16⟩
- Accession number :
- edsair.doi.dedup.....ba555b6895408a9586da5b7d53d1c22a