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The Graphical Studies of the Major Molecular Interactions for Neural Cell Adhesion Molecule (NCAM) Polysialylation by Incorporating Wenxiang Diagram into NMR Spectroscopy
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 23; Pages: 15128
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
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Abstract
- Polysialylation is a process of polysialic acid (polySia) addition to neural cell adhesion molecule (NCAM), which is associated with tumor cell migration and progression in many metastatic cancers and neurocognition. Polysialylation can be catalyzed by two highly homologous mammalian polysialyltransferases (polySTs), ST8Sia II (STX) and ST8Sia IV (PST). It has been proposed that two polybasic domains, polybasic region (PBR) and polysialyltransferase domain (PSTD) in polySTs, are possible binding sites for the intermolecular interactions of polyST–NCAM and polyST–polySia, respectively, as well as the intramolecular interaction of PSTD–PBR. In this study, Chou’s wenxiang diagrams of the PSTD and PBR are used to determine the key amino acids of these intermolecular and intramolecular interactions, and thus it may be helpful for the identification of the crucial amino acids in the polyST and for the understanding of the molecular mechanism of NCAM polysialylation by incorporating the wenxiang diagram and molecular modeling into NMR spectroscopy.
- Subjects :
- Mammals
Magnetic Resonance Spectroscopy
Organic Chemistry
General Medicine
Sialyltransferases
Catalysis
Computer Science Applications
Inorganic Chemistry
Sialic Acids
Animals
Amino Acids
Physical and Theoretical Chemistry
Neural Cell Adhesion Molecules
Molecular Biology
Spectroscopy
NMR
wenxiang diagram
sialic acid (Sia)
polysialic acid (polySia)
polysialyltransferases (polyST)
ST8Sia II (STX)
ST8Sia IV (PST)
neural cell adhesion molecule (NCAM)
polysialyltransferase domain (PSTD)
polybasic region (PBR)
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 23; Pages: 15128
- Accession number :
- edsair.doi.dedup.....ba54afa74d318f0d12c3908e95fedef6
- Full Text :
- https://doi.org/10.3390/ijms232315128