Back to Search
Start Over
Glutaredoxin Deletion Shortens Chronological Life Span in Saccharomyces cerevisiae via ROS-Mediated Ras/PKA Activation
- Source :
- Journal of proteome research. 17(7)
- Publication Year :
- 2018
-
Abstract
- Glutaredoxins (GRXs), small redox proteins that use reduced glutathione as an electron donor, are key components of the cellular antioxidant system. In this study, we used Saccharomyces cerevisiae as a model system to investigate the effects of GRX deletion on yeast chronological life span (CLS). Deletion of either Grx1 or Grx2 shortened yeast CLS. Quantitative proteomics revealed that GRX deletion decreased the expression of stress-response proteins, leading to increased cellular reactive oxygen species accumulation and, subsequently, intracellular acidification. This activated the Ras/protein kinase A (PKA) signaling pathway. Genetic and biochemical analyses demonstrated that Ras/PKA activation decreased stress resistance and increased biosynthesis, requiring yeast cells to grow under unfavorable conditions and resulting in a shortened CLS. Our results provided new insights into mechanisms underlying exacerbation of the aging process by oxidative stress.
- Subjects :
- 0301 basic medicine
Aging
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae
Longevity
medicine.disease_cause
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Glutaredoxin
medicine
Protein kinase A
Glutaredoxins
chemistry.chemical_classification
Reactive oxygen species
030102 biochemistry & molecular biology
biology
General Chemistry
Glutathione
biology.organism_classification
Cyclic AMP-Dependent Protein Kinases
Yeast
Cell biology
Oxidative Stress
030104 developmental biology
chemistry
Protein Biosynthesis
ras Proteins
Signal transduction
Reactive Oxygen Species
Oxidative stress
Gene Deletion
Signal Transduction
Subjects
Details
- ISSN :
- 15353907
- Volume :
- 17
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of proteome research
- Accession number :
- edsair.doi.dedup.....ba44a526e3dc516552ad377b961cad48