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Protein kinase C involved in zymosan-induced release of arachidonic acid and superoxide but not in calcium ionophore-elicited arachidonic acid release or formation of prostaglandin E2 from added arachidonate
- Source :
- Journal of Cellular Biochemistry. 48:288-295
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- Zymosan and phorbol ester induced in liver macrophages the release of arachidonic acid, prostaglandin E2, and superoxide; the calcium ionophore A 23187 elicited a release of arachidonic acid and prostaglandin E2 but not of superoxide, and exogenously added arachidonic acid led to the formation of prostaglandin E2 only. The zymosan- and phorbol-ester-induced release of arachidonic acid, prostaglandin E2, and superoxide was dose-dependently inhibited by staurosporine and K252a, two inhibitors of protein kinase C, and by pretreatment of the cells with phorbol ester which desensitized protein kinase C. The release of arachidonic acid or prostaglandin E2 following the addition of A 23187 or arachidonic acid was not affected by these treatments. Zymosan and phorbol ester but not A 23187 or arachidonic acid induced a translocation of protein kinase C from the cytosol to membranes in intact cells. These results demonstrate an involvement of protein kinase C in the zymosan- and phorbol-ester-induced release of arachidonic acid, prostaglandin E2, and superoxide; the release of arachidonic acid and prostaglandin E2 elicited by A 23187 and the formation of prostaglandin E2 from exogenously added arachidonic acid, however, is independent of an activation of protein kinase C.
- Subjects :
- Male
Kupffer Cells
Blotting, Western
Carbazoles
Arachidonic Acids
Biochemistry
Dinoprostone
Indole Alkaloids
chemistry.chemical_compound
Alkaloids
Phospholipase A2
Superoxides
Discovery and development of cyclooxygenase 2 inhibitors
medicine
Animals
Rats, Wistar
Prostaglandin E2
Protein kinase A
Molecular Biology
Calcimycin
Cells, Cultured
Protein Kinase C
Protein kinase C
biology
Superoxide
Zymosan
Cell Biology
Staurosporine
Rats
chemistry
biology.protein
Tetradecanoylphorbol Acetate
Arachidonic acid
medicine.drug
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....ba350b9fd460635438ca118c17a2934f