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Overexpression of the secreted factor Mig30 expressed in the Spemann organizer impairs morphogenetic movements during Xenopus gastrulation
- Source :
- Mechanisms of Development. 112(1-2):37-51
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- The Spemann organizer secretes several antagonists of growth factors during gastrulation. We describe a novel secreted protein, Mig30, which is expressed in the anterior endomesoderm of the Spemann organizer. Mixer-inducible gene 30 (Mig30) was isolated as a target of Mixer, a homeobox gene required for endoderm development. The Mig30 gene encodes a secreted protein containing a cysteine-rich domain and an immunoglobulin-like domain that belongs to the insulin-like growth factor-binding protein family. Overexpression of Mig30 in the dorsal region results in the retardation of morphogenetic movements during gastrulation and leads to microcephalic embryos. Overexpression of Mig30 also inhibits activin-induced elongation of ectodermal explants without affecting gene expression patterns in mesoderm and endoderm. These results suggest that Mig30 is involved in the regulation of morphogenetic movements during gastrulation in the extracellular space of the Spemann organizer.
- Subjects :
- Mesoderm
Embryology
DNA, Complementary
Indoles
Time Factors
animal structures
Transcription, Genetic
Protein family
Xenopus
Blotting, Western
Molecular Sequence Data
Xenopus Proteins
Open Reading Frames
Endomesoderm
Ectoderm
medicine
Animals
Amino Acid Sequence
Cysteine
RNA, Messenger
In Situ Hybridization
Homeodomain Proteins
biology
Reverse Transcriptase Polymerase Chain Reaction
Organizers, Embryonic
Gene Expression Regulation, Developmental
Nucleic Acid Hybridization
Proteins
Galactosides
Embryo
Gastrula
biology.organism_classification
Immunohistochemistry
Molecular biology
Insulin-Like Growth Factor Binding Proteins
Gastrulation
medicine.anatomical_structure
embryonic structures
Intercellular Signaling Peptides and Proteins
Homeobox
Endoderm
Plasmids
Transcription Factors
Developmental Biology
Subjects
Details
- ISSN :
- 09254773
- Volume :
- 112
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Mechanisms of Development
- Accession number :
- edsair.doi.dedup.....ba29625d10b9345d9cc5a675fb9144ea
- Full Text :
- https://doi.org/10.1016/s0925-4773(01)00638-4