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Development and evaluation of a new recombinant protein vaccine (YidR) against Klebsiella pneumoniae infection
- Source :
- Vaccine. 38:4640-4648
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Vaccination is a promising approach to prevent Klebsiella infection; however, the high heterogeneity of strains is a limiting factor. The best antigenic target for an anti-Klebsiella vaccine should be expressed by all or most of strains. We previously found YidR protein to be highly conserved among K. pneumoniae strains independently of antigen serotype. Therefore, in the present study, we developed a recombinant YidR protein vaccine and evaluated its protective efficacy against lethal challenge with K. pneumoniae in a mouse model. The yidR gene was cloned in Escherichia coli for recombinant expression. The lethal dose (LD100) of K. pneumoniae was determined and lethal challenge was carried out after immunization with recombinant purified YidR. After immunization, the concentration of total serum IgG was significantly higher in YidR-immunized mice than in non-immunized mice, indicating strong induction of antibodies. Mice were challenged with LD100 of K. pneumoniae, and significantly lower murine sepsis and higher body weight were observed in YidR-immunized mice compared to unvaccinated controls. Moreover, ∼90% of YidR-immunized mice survived beyond 10 days of observation, whereas none of the control mice survived past 48 h. The protective effect of YidR recombinant protein vaccine was demonstrated and YidR may be a promising vaccine candidate to prevent klebsiellosis.
- Subjects :
- Klebsiella pneumoniae
030231 tropical medicine
medicine.disease_cause
law.invention
Microbiology
Mice
03 medical and health sciences
0302 clinical medicine
Antigen
law
medicine
Animals
030212 general & internal medicine
Escherichia coli
Mice, Inbred BALB C
General Veterinary
General Immunology and Microbiology
biology
Lethal dose
Public Health, Environmental and Occupational Health
biology.organism_classification
Antibodies, Bacterial
Recombinant Proteins
Klebsiella Infections
Vaccination
Infectious Diseases
Immunization
Immunoglobulin G
Recombinant DNA
biology.protein
Molecular Medicine
Antibody
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....ba253c3f5d05dad45768b4783ab44ec7