Back to Search Start Over

Phospholipid signaling in apoptosis: peroxidation and externalization of phosphatidylserine

Phospholipid signaling in apoptosis: peroxidation and externalization of phosphatidylserine

Authors :
Vladimir A. Tyurin
Anna A. Shvedova
Kazuaki Kawai
James P. Fabisiak
Peter J. Quinn
Nina F. Schor
Choudari Kommineni
Y.Y. Tyurina
Valerian E. Kagan
Source :
Toxicology. 148(2-3)
Publication Year :
2000

Abstract

The role of phospholipids in apoptosis signaling and the relationship between oxidation of phosphatidylserine and its redistribution in the plasma membrane were studied. A novel method for detection of site-specific phospholipid peroxidation based on the use of cis-parinaric acid as a reporter molecule metabolically integrated into membrane phospholipids in living cells was employed. When several tissue culture cell lines and different exogenous oxidants were used, the relationship between the oxidation of phosphatidylserine and apoptosis has been revealed. The plasma membrane was the preferred site of phosphatidylserine oxidation in cells. It was shown that selective oxidation of phosphatidylserine precedes its translocation from the inside to the outside surface of the plasma membrane during apoptosis. A model is proposed in which cytochrome c released from mitochondria by oxidative stress binds to phosphatidylserine located at the cytoplasmic surface of the plasma membrane and induces its oxidation. Interaction of peroxidized phosphatidylserine with aminophospholipid translocase causes inhibition of the enzyme relevant to phosphatidylserine externalization.

Details

ISSN :
0300483X
Volume :
148
Issue :
2-3
Database :
OpenAIRE
Journal :
Toxicology
Accession number :
edsair.doi.dedup.....ba1d13a99007987a063c55fcd9c5757d