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Construction of a genetically engineered microorganism with high tolerance to arsenite and strong arsenite oxidative ability
- Source :
- Journal of Environmental Science and Health, Part A. 45:740-745
- Publication Year :
- 2010
- Publisher :
- Informa UK Limited, 2010.
-
Abstract
- Genetically engineered microorganisms (GEMs) have shown great potential for use in environmental bioremediation. In this study, the TTHB128 and TTHB127 genes, which encode the small and large subunits of arsentie oxidase in Thermus thermophilus HB8, respectively, were cloned into the broad-host-range vector pBBR1MCS-5 to produce the recombinant plasmid, TTHB127-pBBR1MCS-5-TTHB128. This resulted in successful construction of a GEM with high tolerance to arsenite and strong arsenite oxidative ability. Culture of the GEM in media containing arsenite for 28 h resulted in 87.6% of the arsenite being oxidized. Overall, the oxidative ability of the GEM was much stronger than that of the wild type host strain. Gentamicin was necessary to maintain the stability of the recombinant plasmid, TTHB127-pBBR1MCS-5-TTHB128, in the GEM. The oxidative ability of the GEM remained unchanged when it was grown in medium containing gentamicin (60 mg/L) for 30 growth cycles, after which its activity gradually decreased.
- Subjects :
- Environmental Engineering
endocrine system diseases
Arsenites
Genetic Vectors
Oxidative phosphorylation
Biology
Microbiology
law.invention
chemistry.chemical_compound
law
Cloning, Molecular
DNA Primers
Arsenite
chemistry.chemical_classification
Oxidase test
Base Sequence
Organisms, Genetically Modified
Thermus thermophilus
Wild type
General Medicine
biology.organism_classification
Adaptation, Physiological
Genetically modified organism
Enzyme
chemistry
Recombinant DNA
Oxidoreductases
Oxidation-Reduction
Subjects
Details
- ISSN :
- 15324117 and 10934529
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Journal of Environmental Science and Health, Part A
- Accession number :
- edsair.doi.dedup.....ba139dd017bde0e22bc21d1eeb3a9c32