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Molecular portraits of B cell lineage commitment
- Source :
- Proceedings of the National Academy of Sciences. 99:10014-10019
- Publication Year :
- 2002
- Publisher :
- Proceedings of the National Academy of Sciences, 2002.
-
Abstract
- In an attempt to characterize early B cell development including the commitment of progenitor cells to the B cell lineage, we generated and compared genomewide gene expression profiles of human hematopoietic stem cells (HSCs) and pre-B cells (PBCs) by using serial analysis of gene expression. From more than 100,000 serial analysis of gene expression tags collected from human CD34 + HSCs and CD10 + CD19 + PBCs, 42,399 unique transcripts were identified in HSCs but only 16,786 in PBCs, suggesting that more than 60% of transcripts expressed in HSCs were silenced during or after commitment to the B cell lineage. On the other hand, mRNAs of pre-B cell receptor (pre-BCR)-associated genes are virtually missing in HSCs but account for more than 10% of the transcriptome of PBCs, which also show increased expression of apoptosis-related genes. Both concentration of the transcriptional repertoire on pre-BCR-related genes together with marked up-regulation of apoptosis mediators in PBC might reflect selection for the expression of a functional pre-BCR within the bone marrow. Besides known regulator genes of early B cell development such as PAX5 , E2A , and EBF , the most abundantly expressed genes in PBCs include ATM , PDGFRA , SIAH1 , PIM2 , C/EBPB , WNT16 , and TCL1 , the role of which has not been established yet in early B cell development.
- Subjects :
- Transcription, Genetic
Cellular differentiation
Antigens, CD19
B-Lymphocyte Subsets
Antigens, CD34
Biology
CD19
Immunophenotyping
Transcriptome
Antigens, CD
medicine
Humans
RNA, Messenger
Serial analysis of gene expression
Progenitor cell
B cell
Gene Library
Regulation of gene expression
B-Lymphocytes
Multidisciplinary
Cell Differentiation
Biological Sciences
Hematopoietic Stem Cells
Cell biology
medicine.anatomical_structure
Gene Expression Regulation
Cancer research
biology.protein
Neprilysin
Stem cell
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....b9eb655076fa3ad90a6cebe4c66f8044