Back to Search
Start Over
Cadmium Toxicity on Arterioles Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats
- Source :
- International Journal of Environmental Research and Public Health; Volume 3; Issue 4; Pages: 323-328, International Journal of Environmental Research and Public Health, Vol 3, Iss 4, Pp 323-328 (2006)
- Publication Year :
- 2006
- Publisher :
- MDPI AG, 2006.
-
Abstract
- Cadmium (Cd) is frequently used in various industrial applications and is a ubiquitous environmental toxicant, also present in tobacco smoke. An important route of exposure is the circulatory system whereas blood vessels are considered to be main stream organs of Cd toxicity. Our previous results indicate that cadmium chloride (CdCl2) affects mean arterial blood pressure in hypertensive rats. We hypothesized that Cd alters the intracellular calcium transient mechanism, by cadmium-induced stimulation of MAPKs (ERK 1 & 2) which is mediated partially through calcium-dependent PKC mechanism. To investigate this hypothesis, we exposed primary cultures of vascular smooth muscle cells (VSMCs) from wistar kyoto (WKY) and spontaneously hypertensive rats (SHR) to increased concentrations of CdCl2 on cell viability, expression of mitogen-activated protein kinases (MAPKs/ERK 1 & 2), and protein kinase C (PKC) which are activated by Cd in several cell types. The results from these studies indicate that CdCl2 decreased cell viability of both SHR and WKY VSMCs in a concentration dependent-manner. Viability of both cell types decreased 33±5.3 (SHR) and 39±2.3% (WKY) when exposed to 1 μM CdCl2, whereas, 8 and 16 μM reduced viability by 66±3.1 and 62±4.5% in SHR cells. CdCl2 increased ERK 1 & 2 in a biphasic manner with maximum increase occurring when cells are exposed to 1 and 4 μM in SHR VSMCs, whereas, a reduction in ERK 1 and 2 is observed when WKY cells are treated with 2 μM. The results also indicate that CdCl2 increased PKC a/ß in both SHR and WKY VSMCs with a greater increase in expression in SHR VSMCs. In addition, the [Ca2+]i chelator, BAPTA, suppressed the CdCl2 effect, whereas, the PKC inhibitor, GF109203X, reduced the CdCl2 induced-effect on PKC expression. The present studies support the hypothesis that Cd can be a risk factor of hypertension through dysfunction of vascular smooth muscle cells under certain conditions.
- Subjects :
- inorganic chemicals
MAPK/ERK pathway
Cadmium Poisoning
medicine.medical_specialty
Vascular smooth muscle
mitogen-activated protein kinase
Cell Survival
Health, Toxicology and Mutagenesis
ERK 1
Myocytes, Smooth Muscle
ERK 2
lcsh:Medicine
Stimulation
Biology
Cadmium chloride
Muscle, Smooth, Vascular
Calcium in biology
chemistry.chemical_compound
BAPTA
Rats, Inbred SHR
Internal medicine
medicine
vascular smooth muscle cells
Animals
Viability assay
Protein kinase C
Cells, Cultured
Protein Kinase C
Mitogen-Activated Protein Kinase Kinases
Dose-Response Relationship, Drug
lcsh:R
Public Health, Environmental and Occupational Health
Rats
Endocrinology
chemistry
cardiovascular system
Cadmium
Subjects
Details
- ISSN :
- 16604601
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....b9de428c949ff8a9fdd715fa67d838e4