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Effect of exogenous DMNPE-caged ATP on in vitro-matured bovine oocytes prior to parthenogenetic activation, fertilisation and nuclear transfer
- Source :
- Reproduction, fertility, and development. 16(8)
- Publication Year :
- 2004
-
Abstract
- Adenosine triphosphate (ATP) plays an important role during fertilisation of the mammalian oocyte through its ability to alter the frequency and duration of calcium oscillations. It has also been shown that higher ATP levels correlate with increased developmental competence in bovine and human oocytes. During somatic cell nuclear transfer (NT), the incoming nucleus is remodelled extensively, undoubtedly using a variety of ATP-dependent enzymes. The aim of the present study was to determine whether additional exogenous ATP influences activation of parthenogenetic (PA), in vitro-fertilised (IVF) or cloned (NT) in vitro-matured bovine oocytes. Blastocyst development and cell numbers in PA embryos were found to increase in a dose-dependent manner following the photorelease of 0, 50, 100, 500 and 1000 μm DMNPE-caged ATP (adenosine 5′-triphosphate, P3-(1-(4,5-dimethoxy-2-nitrophenyl)ethyl) ester, disodium salt). No cleavage was found following release of 2 and 5 mm DMNPE-caged ATP or with DMNPE-caged ATP (not photoreleased). There were also no differences in blastocyst rates or cell numbers between the control group and groups treated with caged, but not photoreleased, ATP. The addition of exogenous ATP before IVF or to NT couplets did not result in a significant increase in blastocyst development or cell number. Embryo transfer is necessary to determine whether exogenous ATP can positively affect reprogramming, resulting in higher cloned pregnancy rates or live-term births.
- Subjects :
- Nuclear Transfer Techniques
Parthenogenesis
Reproductive technology
Fertilization in Vitro
Biology
chemistry.chemical_compound
Endocrinology
Adenosine Triphosphate
Genetics
medicine
Animals
Blastocyst
Molecular Biology
Fertilisation
Embryo culture
Oocyte
Adenosine
Cell biology
medicine.anatomical_structure
Reproductive Medicine
chemistry
Oocytes
Somatic cell nuclear transfer
Animal Science and Zoology
Cattle
Female
Adenosine triphosphate
Developmental Biology
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 10313613
- Volume :
- 16
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Reproduction, fertility, and development
- Accession number :
- edsair.doi.dedup.....b9c341277aa9e680f75638b389b2199a