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Therapeutic Efficacy of Cord Blood-Derived Mesenchymal Stromal Cells for the Prevention of Acute Graft-Versus-Host Disease in a Xenogenic Mouse Model

Authors :
Christian Beauséjour
Joëlle Gregoire-Gauthier
Geneviève Despars
Mame Massar Dieng
Natalie Patey
François Fontaine
Silvia Selleri
Elie Haddad
Source :
Stem Cells and Development. 21:1616-1626
Publication Year :
2012
Publisher :
Mary Ann Liebert Inc, 2012.

Abstract

Human mesenchymal stromal cells (MSCs) have been successfully utilized for the treatment of refractory graft-versus-host disease (GvHD). Despite the large number of in vitro and in vivo models developed for clarifying their immunomodulatory properties, the mechanism of action of MSCs remains elusive and their efficacy controversial. Here, we tested the ability of cord blood-derived MSCs to alleviate the symptoms of GvHD induced by the injection of human peripheral blood mononuclear cells into NOD/SCID/γc(-) mice. In this in vivo xeno-GvHD model, we demonstrate that a single MSC injection is able to inhibit GvHD in terms of clinical signs and related mortality. We also show that in this model MSCs act by both immunomodulating T-cells and fostering recovery after irradiation. The translational impact of these findings could provide a reliable preclinical model for studying the efficacy, dosage, and time of administration of human MSCs for the prevention of acute GvHD.

Details

ISSN :
15578534 and 15473287
Volume :
21
Database :
OpenAIRE
Journal :
Stem Cells and Development
Accession number :
edsair.doi.dedup.....b9c01781be08e64beea36138b3c314bd