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A metabolic and genomic assessment of sugar fermentation profiles of the thermophilic Thermotogales, Fervidobacterium pennivorans
- Source :
- Extremophiles. 22:965-974
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- A metabolic, genomic and proteomic assessment of Fervidobacterium pennivorans strains was undertaken to clarify the metabolic and genetic capabilities of this Thermotogales species. The type strain Ven5 originally isolated from a hot mud spa in Italy, and a newly isolated strain (DYC) from a hot spring at Ngatamariki, New Zealand, were compared for metabolic and genomic differences. The fermentation profiles of both strains on cellobiose generated similar major end products (acetate, alanine, glutamate, H2, and CO2). The vast majority of end products produced were redox neutral, and carbon balances were in the range of 95–115%. Each strain showed distinct fermentation profiles on sugar substrates. The genome of strain DYC was sequenced and shown to have high sequence similarity and synteny with F. pennivorans Ven5 genome, suggesting they are the same species. The unique genome regions in Ven5, corresponded to genes involved in the Entner–Doudoroff pathway confirming our observation of DYC’s inability to utilize gluconate. Genome analysis was able to elucidate pathways involved in production of the observed end-products with the exception of alanine and glutamate synthesis which were resolved with less clarity due to poor sequence identity and missing critical enzymes within the pathway, respectively.
- Subjects :
- Thermotolerance
0301 basic medicine
030106 microbiology
Cellobiose
Biology
Microbiology
Genome
Substrate Specificity
Gram-Negative Anaerobic Straight, Curved, and Helical Rods
03 medical and health sciences
chemistry.chemical_compound
Gene
Synteny
chemistry.chemical_classification
Strain (chemistry)
Thermophile
General Medicine
030104 developmental biology
Enzyme
chemistry
Biochemistry
Fermentation
Molecular Medicine
Sugars
Genome, Bacterial
Subjects
Details
- ISSN :
- 14334909 and 14310651
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Extremophiles
- Accession number :
- edsair.doi.dedup.....b9b959418b218d5c6b8ae6fb91c37ea0