Back to Search
Start Over
Epstein‐Barr Virus‐encoded Latent Membrane Protein 1 Co‐expresses with Epidermal Growth Factor Receptor in Nasopharyngeal Carcinoma
- Source :
- Japanese Journal of Cancer Research : Gann
- Publication Year :
- 1999
- Publisher :
- Blackwell Publishing Ltd, 1999.
-
Abstract
- Latent membrane protein 1 (LMP-1) is the only Epstein-Barr virus (EBV)-encoded oncogenic protein that has been detected in nasopharyngeal carcinoma (NPC), a cancer that is closely associated with EBV. Previous in-vitro studies have demonstrated that LMP-1 can upregulate epidermal growth factor receptor (EGFR) in epithelial cells. It was not established whether this cellular effect exists in NPC. To assess the association between LMP-1 and EGFR in NPC tissues, 60 NPC specimens were examined by immunohistochemistry using anti-LMP-1 antibody (CS 1-4) and anti-EGFR antibodies (EGFR 1, EGFR 1005). The results revealed that 41 (68.3%) specimens were immunopositive for LMP-1 and 44 (73.3%) specimens over-expressed EGFR. Morphologically, the expressions of LMP-1 and EGFR were homogeneously distributed in the tumor nests. In addition, the correlation between LMP-1 and EGFR was statistically significant (P0.001, chi2 test, d.f. = 1). To elucidate further the correlation between LMP-1 and EGFR in vivo and in situ, an indirect dual immunofluorescence assay was conducted, using secondary antibodies conjugated with fluorescein isothiocyanate (FITC) or indocarbocyanine (Cy3). The results disclosed an intimate co-expression of LMP-1 and EGFR. In summary, the data indicate that over-expression of EGFR is a common phenomenon in NPC, and that EGFR is co-expressed with LMP-1 in NPC. Thus, EBV may play a role in the tumorigenesis of NPC through the effects of LMP-1 and EGFR.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Herpesvirus 4, Human
viruses
Biology
medicine.disease_cause
Immunofluorescence
Article
Viral Matrix Proteins
chemistry.chemical_compound
Cell surface receptor
Epidermal growth factor
hemic and lymphatic diseases
Latent membrane protein 1
medicine
otorhinolaryngologic diseases
Humans
Epidermal growth factor receptor
Fluorescein isothiocyanate
Fluorescent Antibody Technique, Indirect
Co‐expression
Indirect dual immunofluorescence
medicine.diagnostic_test
Nasopharyngeal Neoplasms
Oncogene Proteins, Viral
medicine.disease
Epstein–Barr virus
Immunohistochemistry
ErbB Receptors
stomatognathic diseases
Oncology
Nasopharyngeal carcinoma
chemistry
Cancer research
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 18764673 and 09105050
- Volume :
- 90
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Japanese Journal of Cancer Research : Gann
- Accession number :
- edsair.doi.dedup.....b9a9d7a9ad5bc1ff0027048c6f4aa1d3