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β-Aminoisobutyric acid, L-BAIBA, protects PC12 cells from hydrogen peroxide-induced oxidative stress and apoptosis via activation of the AMPK and PI3K/Akt pathway
- Source :
- IBRO Neuroscience Reports, IBRO Neuroscience Reports, Vol 12, Iss, Pp 65-72 (2022)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- β-Aminoisobutyric acid (BAIBA) is a myokine that is secreted from skeletal muscles by the exercise. Recently, increasing evidence has suggested the multifocal physiological activities of BAIBA. In this study, we investigated whether L-BAIBA has protective effects on rat pheochromocytoma (PC12) cells. Cultured PC12 cells were stimulated with L-BAIBA. Western blot analyses revealed that L-BAIBA stimulation significantly increased the phosphorylation of AMPK and Akt. In contrast, no effect was observed on neurite outgrowth by L-BAIBA. To investigate the effects of L-BAIBA on oxidative stress, PC 12 cells were exposed to hydrogen peroxide (H2O2) with and without L-BAIBA. Hydrogen peroxide significantly increased reactive oxygen species (ROS) production and apoptosis in PC12 cells. Pretreatment with L-BAIBA suppressed H2O2-induced ROS production and apoptosis, which was abolished by the inhibition of AMPK by compound C. On the other hand, the inhibitory effects of L-BAIBA on oxidative stress-induced apoptosis were abolished by the inhibition of both AMPK and PI3K/Akt. In conclusion, we demonstrated that L-BAIBA confers protection against oxidative stress in PC12 cells by activating the AMPK and PI3K/Akt pathways. These results suggest that L-BAIBA may play a crucial role on protection of neuron-like cells and become a pharmacological agent to treat neuronal diseases.
- Subjects :
- BAIBA, β-Aminoisobutyric acid
AMPK
PI3K/Akt
PPARγ, peroxisome proliferator-activated receptor gamma
TUNEL, TdT-mediated dUTP nick-end labeling
General Neuroscience
Neurosciences. Biological psychiatry. Neuropsychiatry
Neuron
MRGPRD, Mas-related G protein-coupled receptor type D
Hydrogen peroxide
DMEM, Dulbecco’s modified Eagle’s medium
ROS, reactive oxygen species
FBS, fetal bovine serum
Oxidative stress
β-Aminoisobutyric acid (BAIBA)
FITC, fluorescein isothiocyanate
PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1-alpha
GPCR, G protein-coupled receptor
RC321-571
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 26672421
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- IBRO Neuroscience Reports
- Accession number :
- edsair.doi.dedup.....b998bc57e7f2d0b2c72bfab5039075a5