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Devitalized Stem Cell Microsheets for Sustainable Release of Osteogenic and Vasculogenic Growth Factors and Regulation of Anti‐Inflammatory Immune Response
- Source :
- Advanced Biosystems. 1:1600011
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- The objective of this work was to investigate the effect of devitalized human mesenchymal stem cells (hMSCs) and endothelial colony-forming cells (ECFCs) seeded on mineralized nanofiber microsheets on protein release, osteogenesis, vasculogenesis, and macrophage polarization. Calcium phosphate nanocrystals were grown on the surface of aligned, functionalized nanofiber microsheets. The microsheets were seeded with hMSCs, ECFCs, or a mixture of hMSCs+ECFCs, cultured for cell attachment, differentiated to the osteogenic or vasculogenic lineage, and devitalized by lyophilization. The release kinetic of total protein, bone morphogenetic protein-2 (BMP2), and vascular endothelial growth factor (VEGF) from the devitalized microsheets was measured. Next, hMSCs and/or ECFCs were seeded on the devitalized cell microsheets and cultured in the absence of osteo-/vasculo-inductive factors to determine the effect of devitalized cell microsheets on hMSC/ECFC differentiation. Human macrophages were seeded on the microsheets to determine the effect of devitalized cells on macrophage polarization. Based on the results, devitalized undifferentiated hMSC and vasculogenic-differentiated ECFC microsheets had highest sustained release of BMP2 and VEGF, respectively. The devitalized hMSC microsheets did not affect M2 macrophage polarization while vascular-differentiated, devitalized ECFC microsheets did not affect M1 polarization. Both groups stimulated higher M2 macrophage polarization compared to M1.
- Subjects :
- 0301 basic medicine
Chemistry
Cell
Mesenchymal stem cell
Biomedical Engineering
Macrophage polarization
02 engineering and technology
equipment and supplies
021001 nanoscience & nanotechnology
M2 Macrophage
Bone morphogenetic protein 2
Article
General Biochemistry, Genetics and Molecular Biology
Cell biology
Biomaterials
Vascular endothelial growth factor
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Vasculogenesis
medicine.anatomical_structure
Immunology
medicine
Stem cell
0210 nano-technology
Subjects
Details
- ISSN :
- 23667478
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Advanced Biosystems
- Accession number :
- edsair.doi.dedup.....b98a1fc4eb7484d0bea1ce757cbb8940