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Spray-dried plasma attenuates inflammation and lethargic behaviors of pregnant mice caused by lipopolysaccharide
- Source :
- PLoS ONE, Vol 13, Iss 9, p e0203427 (2018), PloS one, vol 13, iss 9
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- This study evaluated whether dietary spray-dried plasma (SDP) can ameliorate inflammation, lethargic behaviors, and impairment of reproduction caused by lipopolysaccharide (LPS) challenge during late pregnancy. Two experiments were conducted with 125 mated female mice (C57BL/6 strain) in each experiment. All mice were shipped from a vendor on the gestation day (GD) 1 and arrived at the laboratory on GD 3. Mice were randomly assigned to dietary treatments with or without 8% SDP in the diet. On GD 17, mice determined pregnant by BW and abdomen shape were randomly assigned to intraperitoneal injections with or without 2 μg LPS. In experiment 1, 17 mice (26.7 ± 1.7 g BW) were identified pregnant and euthanized 6 h after the LPS challenge to measure inflammatory responses in uterus and placenta. In experiment 2, 44 mice (26.0 ± 1.6 g BW) were identified pregnant and euthanized 24 h after the LPS challenge to assess behavior and late-term pregnancy loss. Growth performance and reproductive responses, such as loss of pregnancy, percentage of fetal death, and etc., were measured in all pregnant mice. The LPS challenge increased (P < 0.05) uterine and placental tumor necrosis factor-α and interferon-γ, late-term pregnancy loss, and lethargy score, and decreased (P < 0.05) uterine transforming growth factor-β1, moving time and number of rearing, and growth and feed intake. The SDP decreased (P < 0.05) concentrations of both pro-inflammatory and anti-inflammatory cytokines in one or both tissues, and the lethargy score, and increased (P < 0.05) moving time and number of rearing, growth of pregnant mice, and fetal weight. However, the SDP did not affect late-term pregnancy loss caused by the LPS challenge. Consequently, dietary SDP attenuated acute inflammation and lethargic behaviors of pregnant mice caused by the LPS challenge, but did not affect late-term pregnancy loss after the acute inflammation.
- Subjects :
- Lethargy
Lipopolysaccharides
0301 basic medicine
medicine.medical_specialty
Lipopolysaccharide
General Science & Technology
Uterus
lcsh:Medicine
Inflammation
Reproductive health and childbirth
Mice
Plasma
03 medical and health sciences
chemistry.chemical_compound
Pregnancy
Internal medicine
Placenta
medicine
2.1 Biological and endogenous factors
Animals
Aetiology
lcsh:Science
Nutrition
Multidisciplinary
business.industry
Contraception/Reproduction
Prevention
lcsh:R
0402 animal and dairy science
04 agricultural and veterinary sciences
medicine.disease
040201 dairy & animal science
Pregnancy Complications
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Dietary Supplements
Gestation
Female
lcsh:Q
Tumor necrosis factor alpha
medicine.symptom
business
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....b96bff499b2435d78fa38f6f796bfe81