Back to Search
Start Over
Induction of oxidative stress related responses inArabidopsis thalianafollowing uranium exposure
- Source :
- Radioprotection. 44:191-196
- Publication Year :
- 2009
- Publisher :
- EDP Sciences, 2009.
-
Abstract
- The reactive oxygen species (ROS)-signaling pathway is very important in heavy metal toxicity. Induction of the antioxidative defense mechanism, comprising ROS-scavenging enzymes and metabolites, in plants after environmental uranium contamination has been insufficiently studied in the past. This study aimed to analyze oxidative stress related responses in Arabidopsis thaliana after uranium exposure. Seventeen-day-old seedlings were exposed to 0, 0.1, 1, 10 and 100 μM uranium for 3 days. After exposure to 100 μM uranium, a decrease in fresh weight for leaves and roots was observed, leaves colored anthocyanous and roots were stunted and yellow. To reveal the importance of oxidative stress in uranium toxicity, alterations in ROS-scavenging enzymes were studied at protein and transcriptional level. Superoxide dismutase (SOD) capacities increased in leaves and roots after exposure to 100 μM uranium but no differences were observed for catalase (CAT) capacities. Transcript levels of different SODs located at various cellular compartments were affected depending on the place of action. Gene expression of CAT in leaves and roots was also affected after uranium exposure. Results indicate that oxidative stress plays an important role in uranium toxicity but suggest that plant responses differ for leaves and roots.
- Subjects :
- inorganic chemicals
Health, Toxicology and Mutagenesis
chemistry.chemical_element
Metal toxicity
medicine.disease_cause
complex mixtures
Toxicology
Superoxide dismutase
medicine
Arabidopsis thaliana
Safety, Risk, Reliability and Quality
Waste Management and Disposal
chemistry.chemical_classification
Reactive oxygen species
biology
Renewable Energy, Sustainability and the Environment
technology, industry, and agriculture
Public Health, Environmental and Occupational Health
Uranium
biology.organism_classification
Nuclear Energy and Engineering
Biochemistry
chemistry
Catalase
Toxicity
biology.protein
Oxidative stress
Subjects
Details
- ISSN :
- 1769700X and 00338451
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Radioprotection
- Accession number :
- edsair.doi.dedup.....b958da860f87cb89d3d4438df1e1a461