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Unique features of flight muscles mitochondria of honey bees (Apis mellifera L.)
- Source :
- Archives of insect biochemistry and physiology. 102(1)
- Publication Year :
- 2019
-
Abstract
- Honey bees Apis mellifera L. are one of the most studied insect species due to their economic importance. The interest in studying honey bees chiefly stems from the recent rapid decrease in their world population, which has become a problem of food security. Nevertheless, there are no systemic studies on the properties of the mitochondria of honey bee flight muscles. We conducted a research of the mitochondria of the flight muscles of A. mellifera L. The influence of various organic substrates on mitochondrial respiration in the presence or absence of adenosine diphosphate (ADP) was investigated. We demonstrated that pyruvate is the optimal substrate for the coupled respiration. A combination of pyruvate and glutamate is required for the maximal respiration rate. We also show that succinate oxidation does not support the oxidative phosphorylation and the generation of membrane potential. We also studied the production of reactive oxygen species by isolated mitochondria. The greatest production of H2 O2 (as a percentage of the rate of oxygen consumed) in the absence of ADP was observed during the respiration supported by α-glycerophosphate, malate, and a combination of malate with another NAD-linked substrate. We showed that honey bee flight muscle mitochondria are unable to uptake Ca2+ -ions. We also show that bee mitochondria are able to oxidize the respiration substrates effectively at the temperature of 50°С compared to Bombus terrestris mitochondria, which were more adapted to lower temperatures.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Male
Physiology
media_common.quotation_subject
Cell Respiration
Insect
Oxidative phosphorylation
Mitochondrion
01 natural sciences
Biochemistry
Membrane Potentials
03 medical and health sciences
chemistry.chemical_compound
Mice
Respiration
Animals
media_common
biology
Muscles
fungi
Temperature
General Medicine
Honey bee
Hydrogen Peroxide
Bees
biology.organism_classification
Mitochondria, Muscle
010602 entomology
Adenosine diphosphate
030104 developmental biology
chemistry
Insect Science
Bombus terrestris
Flight, Animal
behavior and behavior mechanisms
Calcium
Female
Respiration rate
Subjects
Details
- ISSN :
- 15206327
- Volume :
- 102
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Archives of insect biochemistry and physiology
- Accession number :
- edsair.doi.dedup.....b93f5d93092ad8098e193077bcf89dae