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Differential expression of human leukocyte antigen-G (HLA-G) messenger RNAs and proteins in normal human prostate and prostatic adenocarcinoma
- Source :
- Journal of Reproductive Immunology. 71:75-86
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Human leukocyte antigen-G (HLA-G) is a major histocompatibility complex class Ib gene expressed in normal organs and in some tumors. The glycoproteins encoded by this gene are best known for their immunosuppressive properties. Because isoform-specific expression of HLA-G in male reproductive organs has not been reported, we investigated HLA-G1, -G2, -G5, -G6 mRNAs and proteins in four-to-five samples of normal prostate glands, prostates with benign prostatic hyperplasia and prostate adenocarcinomas using RT-PCR and immunohistochemistry. All tissues contained HLA-G1, -G2, -G5 and -G6 specific mRNAs, but only HLA-G5 protein was detectable. In normal prostate glands, HLA-G5 protein was prominent in the cytoplasm of tubuloglandular epithelia and in glandular secretions. Staining was reduced in samples of benign prostatic hyperplasia but remained localized to the cytoplasm of glandular epithelia and secretions. In prostatic adenocarcinomas, HLA-G5 protein was detectable mainly in the secretions. Thus, HLA-G5 but not HLA-G1, -G2 or -G6 is produced in the normal prostate and is present in prostatic secretions. In addition, normal cellular localization is disturbed in benign and malignant prostatic adenocarcinomas. The results are consistent with this molecule may influencing female immune receptivity to sperm and suggest that such immunosuppression could be disturbed in men with prostatic adenocarcinomas.
- Subjects :
- Male
PCA3
Pathology
medicine.medical_specialty
Immunology
Human leukocyte antigen
Adenocarcinoma
Biology
HLA Antigens
Prostate
HLA-G
Leukocytes
medicine
Humans
Immunology and Allergy
RNA, Messenger
Cellular localization
HLA-G Antigens
Histocompatibility Antigens Class I
Prostatic Neoplasms
Obstetrics and Gynecology
Hyperplasia
medicine.disease
Immunohistochemistry
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Reproductive Medicine
Health
Subjects
Details
- ISSN :
- 01650378
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Reproductive Immunology
- Accession number :
- edsair.doi.dedup.....b93f214b93d7345e4d7a7aeffcab72b1