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Increased expression of deoxyribonucleic acid methyltransferase gene in human astrocytic tumors
- Source :
- Neurologia medico-chirurgica. 40(11)
- Publication Year :
- 2000
-
Abstract
- 金沢大学医薬保健研究域医学系<br />金沢大学附属病院脳神経外科<br />The relationship between the grade of astrocytic tumor and the expression of deoxyribonucleic acid methyltransferase (DNA-MTase) gene was examined. The levels of DNA-MTase messenger ribonucleic acid (mRNA) were measured by semiquantitative reverse transcriptase-polymerase chain reaction in surgical specimens from 12 astrocytic tumors (4 astrocytomas, 6 anaplastic astrocytomas, and 2 glioblastomas) and two normal brain tissues, and in four glioma cell lines. Compared to normal brain tissues, the levels of DNA-MTase mRNA were increased by 16- to 55-fold in low grade astrocytomas, and significantly increased by 200- to 4500-fold in high grade astrocytomas (anaplastic astrocytomas and glioblastomas) and more than 4500-fold in glioma cell lines. In situ hybridization with paraffin-embedded surgical specimens of human astrocytic tumors showed DNA-MTase mRNA was abundantly expressed in high grade astrocytomas. The detection of increased DNA-MTase expression in astrocytic tumor indicates involvement in the tumorigenesis and suggests that blocking of this change with specific inhibitors may offer new therapeutic strategies for malignant astrocytic tumors.
- Subjects :
- Methyltransferase
In situ hybridization
Astrocytoma
In Vitro Techniques
medicine.disease_cause
Glioma
Tumor Cells, Cultured
Medicine
Humans
neoplasms
In Situ Hybridization
tRNA Methyltransferases
business.industry
Astrocytic Tumor
Brain Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
DNA, Neoplasm
medicine.disease
nervous system diseases
TRNA Methyltransferases
Deoxyribonucleic acid methyltransferase
Gene Expression Regulation, Neoplastic
Blotting, Southern
Cancer research
Surgery
Neurology (clinical)
business
Carcinogenesis
Semiquantitative reverse transcriptase-polymerase chain reaction
Glioblastoma
Anaplastic astrocytoma
Subjects
Details
- ISSN :
- 04708105
- Volume :
- 40
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Neurologia medico-chirurgica
- Accession number :
- edsair.doi.dedup.....b932953d54837fa3f30e0772fd9e3b3f