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Simultaneous electrospin–electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration
- Source :
- Acta Biomaterialia. 6:4100-4109
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin–electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy and with a universal tensile machine, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was determined by culturing human foetal osteoblasts and investigating the proliferation, alkaline phosphatase (ALP) activity and mineralization of cells. The results of cell proliferation, ALP activity and FESEM studies revealed that the combination of electrospinning of gelatin and electrospraying of HA yielded biocomposite nanofibrous scaffolds with enhanced performances in terms of better cell proliferation, increased ALP activity and enhanced mineralization, making them potential substrates for bone tissue regeneration.
- Subjects :
- Bone Regeneration
Materials science
food.ingredient
Nanofibers
Biomedical Engineering
Bone tissue
Biochemistry
Mineralization (biology)
Gelatin
Bone and Bones
Biomaterials
Extracellular matrix
Calcification, Physiologic
Fetus
food
Coated Materials, Biocompatible
Materials Testing
Cell Adhesion
medicine
Animals
Humans
Fourier transform infrared spectroscopy
Molecular Biology
Cells, Cultured
Cell Proliferation
Osteoblasts
Tissue Scaffolds
Electrochemical Techniques
General Medicine
Electrospinning
Durapatite
medicine.anatomical_structure
Alkaline phosphatase
Biocomposite
Biotechnology
Biomedical engineering
Subjects
Details
- ISSN :
- 17427061
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Acta Biomaterialia
- Accession number :
- edsair.doi.dedup.....b911aef9bce7b20baf71aefa6d57585b
- Full Text :
- https://doi.org/10.1016/j.actbio.2010.05.001