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Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities
- Source :
- Biosensorsbioelectronics. 165
- Publication Year :
- 2020
-
Abstract
- To date, 55% of inhabitants of the developing world still live in rural regions and they have a very urgent need to improve the level of disease diagnosis, especially in resource-limited settings. Herein, we present a microfluidic system that centrifuges whole blood and quantifies contained biomarkers in a rapid, feasible and integrated way. Such a device provides a blood-in/answer-out capability and is small enough to be carried by any individual in any environment. The successful integration a hand-powered centrifuge and immunoassay unit within a rotational paper-based device allows for diagnostic application by untrained users and in environments where access to electricity cannot be assumed. In addition, the low cost (~$ 0.5), light weight and small instrumental footprint make the device ideally suited for rapid on-site detection. To validate the applicability of the system in a clinical diagnostic testing, we successfully perform enzyme-linked immunosorbent assay (ELISA) analysis of carcinoembryonic antigen and alpha fetoprotein from human blood samples. We expect that this powerful platform technology will provide the opportunities for point-of-care diagnosis in resource-limited settings.
- Subjects :
- 2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19)
Computer science
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Point-of-Care Systems
Microfluidics
Biomedical Engineering
Biophysics
Centrifugation
02 engineering and technology
Biosensing Techniques
01 natural sciences
Electrochemistry
Humans
Immunoassay
Human blood
business.industry
010401 analytical chemistry
Diagnostic test
General Medicine
Paper based
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
0104 chemical sciences
Embedded system
0210 nano-technology
business
Biotechnology
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 165
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....b8ff437a7b784013130e588a65ec384f