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Peptide coatings enhance keratinocyte attachment towards improving the peri-implant mucosal seal
- Source :
- Biomaterials Science. 6:1936-1945
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- There is a critical need for preventing peri-implantitis as its prevalence has increased and dental implants lack features to prevent it. Research strategies to prevent peri-implantitis have focused on modifying dental implants to incorporate different antimicrobial agents. An alternative strategy consists of barring the expansion of the biofilm subgingivally by forming a long-lasting permucosal seal between the soft tissue and the implant surface. Here, we innovatively biofunctionalized titanium with bioinspired peptide coatings to strengthen biological interactions between epithelial cells and the titanium surface. We selected laminin 332- and ameloblastin-derived peptides (Lam, Ambn). Laminin 332 participates in the formation of hemidesmosomes by keratinocytes and promotes epithelial attachment around teeth; and ameloblastin, an enamel derived protein, is involved in tissue regeneration events following disruption of the periodontium. Lam, Ambn or combinations of both peptides were covalently immobilized on titanium discs. Successful immobilization of the peptides was confirmed by contact angle goniometry, X-ray photoelectron spectroscopy and fluorescent labelling of the peptides. Additionally, we confirmed the mechanical and thermochemical stability of the peptides on Ti substrates. Proliferation and hemidesmosome formation of human keratinocytes (TERT-2/OKF-6) were assessed by immunofluorescence labelling. The peptide-coated surfaces increased cell proliferation for up to 48 h in culture compared to control surfaces. Most importantly, formation of hemidesmosomes by keratinocytes was significantly increased on surfaces coated with Ambn + Lam peptides compared to control (p < 0.01) and monopeptide coatings (p < 0.005). Together, these results support the Ambn + Lam multipeptide coating as a promising candidate for inducing a permucosal seal around dental implants.
- Subjects :
- Keratinocytes
Surface Properties
Biomedical Engineering
Peptide
02 engineering and technology
Article
03 medical and health sciences
0302 clinical medicine
Coated Materials, Biocompatible
Dental Enamel Proteins
Laminin
Cell Adhesion
medicine
Humans
General Materials Science
AMBN
Amino Acid Sequence
Cell Line, Transformed
Cell Proliferation
Dental Implants
Titanium
chemistry.chemical_classification
biology
Hemidesmosome
030206 dentistry
Periodontium
Hemidesmosomes
021001 nanoscience & nanotechnology
Epithelial Attachment
Peri-Implantitis
Cell biology
stomatognathic diseases
Immobilized Proteins
medicine.anatomical_structure
chemistry
biology.protein
Implant
Peptides
0210 nano-technology
Keratinocyte
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 20474849 and 20474830
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Biomaterials Science
- Accession number :
- edsair.doi.dedup.....b8f48916161bc750dd74f7fdf3ab9dc8