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Telomere position effect is regulated by heterochromatin-associated proteins and NkuA in Aspergillus nidulans
- Source :
- Microbiology
- Publication Year :
- 2010
- Publisher :
- Society for General Microbiology, 2010.
-
Abstract
- Gene-silencing mechanisms are being shown to be associated with an increasing number of fungal developmental processes. Telomere position effect (TPE) is a eukaryotic phenomenon resulting in gene repression in areas immediately adjacent to telomere caps. Here, TPE is shown to regulate expression of transgenes on the left arm of chromosome III and the right arm of chromosome VI inAspergillus nidulans. Phenotypes found to be associated with transgene repression included reduction in radial growth and the absence of sexual spores; however, these pleiotropic phenotypes were remedied when cultures were grown on media with appropriate supplementation. Simple radial growth and ascosporogenesis assays provided insights into the mechanism of TPE, including a means to determine its extent. These experiments revealed that the KU70 homologue (NkuA) and the heterochromatin-associated proteins HepA, ClrD and HdaA were partially required for transgene silencing. This study indicates that TPE extends at least 30 kb on chromosome III, suggesting that this phenomenon may be important for gene regulation in subtelomeric regions ofA. nidulans.
- Subjects :
- Heterochromatin
Transgene
Microbiology
Aspergillus nidulans
Fungal Proteins
03 medical and health sciences
Gene Expression Regulation, Fungal
Gene Silencing
Psychological repression
Ku Autoantigen
030304 developmental biology
Genetics
Regulation of gene expression
0303 health sciences
Fungal protein
biology
030306 microbiology
Antigens, Nuclear
Telomere
biology.organism_classification
DNA-Binding Proteins
Position effect
Cell and Molecular Biology of Microbes
Subjects
Details
- Language :
- English
- ISSN :
- 14652080 and 13500872
- Volume :
- 156
- Issue :
- Pt 12
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....b8e89fef5295a0b48415ef14530a531d