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The occurrence and formation of monoterpenes in herbivoredamaged poplar roots
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
- Publication Year :
- 2018
-
Abstract
- Volatiles are often released upon herbivory as plant defense compounds. While the formation of volatiles above-ground has been intensively studied, little is known about herbivore-induced root volatiles. Here, we show that cockchafer larvae-damaged roots of Populus trichocarpa and P. nigra release a mixture of monoterpenes, including (−)-α-pinene, (−)-camphene, (−)-β-pinene, p-cymene, and 1,8-cineole. Three terpene synthases, PtTPS16 and PtTPS21 from P. trichocarpa and PnTPS4 from P. nigra, could be identified and characterized in vitro. PnTPS4 was found to produce 1,8-cineole as sole product. PtTPS16 and PtTPS21, although highly similar to each other, showed different product specificities and produced γ-terpinene and a mixture of (−)-camphene, (−)-α-pinene, (−)-β-pinene, and (−)-limonene, respectively. Four active site residues were found to determine the different product specificities of the two enzymes. The expression profiles of PtTPS16, PtTPS21, and PnTPS4 in undamaged and herbivore-damaged poplar roots generally matched the emission pattern of monoterpenes, indicating that monoterpene emission in roots is mainly determined at the gene transcript level. Bioassays with Phytophtora cactorum (Oomycetes) revealed inhibitory effects of vapor-phase 1,8-cineole and (−)-β-pinene on the growth of this important plant pathogen. Thus herbivore-induced volatile monoterpenes may have a role in defense against pathogens that cause secondary infections after root wounding.
- Subjects :
- Phytophthora
0106 biological sciences
0301 basic medicine
Populus trichocarpa
Secondary infection
Monoterpene
lcsh:Medicine
Plant Roots
01 natural sciences
Article
Terpene
03 medical and health sciences
Gene Expression Regulation, Plant
Catalytic Domain
Botany
Plant defense against herbivory
Animals
Bioassay
Herbivory
lcsh:Science
Plant Diseases
Plant Proteins
Alkyl and Aryl Transferases
Multidisciplinary
Cockchafer
biology
Plant Extracts
Chemistry
lcsh:R
biology.organism_classification
Terpenoid
Coleoptera
Populus
030104 developmental biology
Monoterpenes
lcsh:Q
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
- Accession number :
- edsair.doi.dedup.....b8cdda8af14bab54654ee84939500159