Back to Search
Start Over
Immobilization of Arsenite and Ferric Iron by Acidithiobacillus ferrooxidans and Its Relevance to Acid Mine Drainage
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (10), pp.6165-6173. ⟨10.1128/AEM.69.10.6165-6173.2003⟩, Applied and Environmental Microbiology, 2003, 69 (10), pp.6165-6173. ⟨10.1128/AEM.69.10.6165-6173.2003⟩
- Publication Year :
- 2003
- Publisher :
- HAL CCSD, 2003.
-
Abstract
- Weathering of the As-rich pyrite-rich tailings of the abandoned mining site of Carnoulès (southeastern France) results in the formation of acid waters heavily loaded with arsenic. Dissolved arsenic present in the seepage waters precipitates within a few meters from the bottom of the tailing dam in the presence of microorganisms. An Acidithiobacillus ferrooxidans strain, referred to as CC1, was isolated from the effluents. This strain was able to remove arsenic from a defined synthetic medium only when grown on ferrous iron. This A. ferrooxidans strain did not oxidize arsenite to arsenate directly or indirectly. Strain CC1 precipitated arsenic unexpectedly as arsenite but not arsenate, with ferric iron produced by its energy metabolism. Furthermore, arsenite was almost not found adsorbed on jarosite but associated with a poorly ordered schwertmannite. Arsenate is known to efficiently precipitate with ferric iron and sulfate in the form of more or less ordered schwertmannite, depending on the sulfur-to-arsenic ratio. Our data demonstrate that the coprecipitation of arsenite with schwertmannite also appears as a potential mechanism of arsenite removal in heavily contaminated acid waters. The removal of arsenite by coprecipitation with ferric iron appears to be a common property of the A. ferrooxidans species, as such a feature was observed with one private and three collection strains, one of which was the type strain.
- Subjects :
- DNA, Bacterial
inorganic chemicals
Arsenites
Acidithiobacillus
Molecular Sequence Data
Inorganic chemistry
chemistry.chemical_element
engineering.material
Ferric Compounds
Applied Microbiology and Biotechnology
Mining
Arsenic
Ferrous
[SHS]Humanities and Social Sciences
chemistry.chemical_compound
RNA, Ribosomal, 16S
DNA, Ribosomal Spacer
Jarosite
Arsenite
MESH: Acidithiobacillus / chemistry* Acidithiobacillus / growth & development Acidithiobacillus / metabolism Arsenic / metabolism* Arsenites / chemistry* Arsenites / metabolism Culture Media DNA, Bacterial / analysis DNA, Ribosomal Spacer / analysis Ferric Compounds / chemistry* Ferric Compounds / metabolism Hydrogen-Ion Concentration Mining* Molecular Sequence Data RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 23S / genetics Sequence Analysis, DNA Water Pollutants, Chemical
Ecology
biology
Chemistry
[SDE.IE]Environmental Sciences/Environmental Engineering
Schwertmannite
Arsenate
Sequence Analysis, DNA
Hydrogen-Ion Concentration
biology.organism_classification
Acid mine drainage
Geomicrobiology
Culture Media
RNA, Ribosomal, 23S
Environmental chemistry
engineering
[SHS] Humanities and Social Sciences
[SDE.IE] Environmental Sciences/Environmental Engineering
Water Pollutants, Chemical
Food Science
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 00992240 and 10985336
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (10), pp.6165-6173. ⟨10.1128/AEM.69.10.6165-6173.2003⟩, Applied and Environmental Microbiology, 2003, 69 (10), pp.6165-6173. ⟨10.1128/AEM.69.10.6165-6173.2003⟩
- Accession number :
- edsair.doi.dedup.....b8c05ce01220439bbfee5651c48c6b73