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Intervertebral disc degeneration in warmblood horses: Histological and biochemical characterization

Authors :
Bergmann, Wilhelmina
de Lest, Chris van
Plomp, Saskia
Vernooij, Johannes C M
Wijnberg, Inge D
Back, Willem
Gröne, Andrea
Delany, Mark W
Caliskan, Nermin
Tryfonidou, Marianna A
Grinwis, Guy C M
Dep Pathobiologie
VPDC pathologie
dPB CR
Equine Musculoskeletal Biology
Veterinaire biochemie
dB&C FR-RMSC RMSC
dES RMSC
CS_Locomotion
FAH Evidence based Veterinary Medicine
dFAH AVR
Equine Internal Medicine
dES AVR
CS_Welfare & emerging diseases
VP pathologie
dPB I&I
Chirurgie
dCSCA RMSC-1
Veterinair Pathologisch Diagnostisch Cnt
LS Pathologie
Dep Pathobiologie
VPDC pathologie
dPB CR
Equine Musculoskeletal Biology
Veterinaire biochemie
dB&C FR-RMSC RMSC
dES RMSC
FAH Evidence based Veterinary Medicine
dFAH AVR
Equine Internal Medicine
dES AVR
VP pathologie
dPB I&I
Chirurgie
dCSCA RMSC-1
CS_Locomotion
CS_Welfare & emerging diseases
Veterinair Pathologisch Diagnostisch Cnt
LS Pathologie
Source :
Veterinary Pathology, 59(2), 284. SAGE Publications Ltd
Publication Year :
2022

Abstract

Gross morphology of healthy and degenerated intervertebral discs (IVDs) is largely similar in horses as in dogs and humans. For further comparison, the biochemical composition and the histological and biochemical changes with age and degeneration were analyzed in 41 warmblood horses. From 33 horses, 139 discs and 2 fetal vertebral columns were evaluated and scored histologically. From 13 horses, 73 IVDs were assessed for hydration, DNA, glycosaminoglycans, total collagen, hydroxyl-lysyl-pyridinoline, hydroxylysine, and advanced glycation end-product (AGE) content. From 7 horses, 20 discs were assessed for aggrecan, fibronectin, and collagen type 1 and 2 content. Histologically, tearing of the nucleus pulposus (NP) and cervical annulus fibrosus (AF), and total histological score (tearing and vascular proliferation of the AF, and chondroid metaplasia, chondrocyte-like cell proliferation, presence of notochordal cells, matrix staining, and tearing of the NP) correlated with gross degeneration. Notochordal cells were not seen in IVDs of horses. Age and gross degeneration were positively correlated with AGEs and a fibrotic phenotype, explaining gross degenerative changes. In contrast to dogs and humans, there was no consistent difference in glycosaminoglycan content and hydration between AF and NP, nor decrease of these variables with age or degeneration. Hydroxylysine decrease and collagen 1 and AGEs increase were most prominent in the NP, suggesting degeneration started in the AP. In caudal cervical NPs, AGE deposition was significantly increased in grossly normal IVDs and total collagen significantly increased with age, suggesting increased biomechanical stress and likelihood for spinal disease in this part of the vertebral column.

Details

Language :
English
ISSN :
03009858
Database :
OpenAIRE
Journal :
Veterinary Pathology, 59(2), 284. SAGE Publications Ltd
Accession number :
edsair.doi.dedup.....b8a98e7e2a3548898f71fdf1b70ddc5d