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FMN-dependent oligomerization of putative lactate oxidase from Pediococcus acidilactici
- Source :
- PLoS ONE, 'PloS One ', vol: 15, pages: e0223870-1-e0223870-16 (2020), PLoS ONE, Vol 15, Iss 2, p e0223870 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science, 2020.
-
Abstract
- Lactate oxidases belong to a group of FMN-dependent enzymes and they catalyze a conversion of lactate to pyruvate with a release of hydrogen peroxide. Hydrogen peroxide is also utilized as a read out in biosensors to quantitate lactate levels in biological samples.Aerococcus viridanslactate oxidase is the best characterized lactate oxidase and our knowledge of lactate oxidases relies largely to studies conducted with that particular enzyme.Pediococcus acidilacticilactate oxidase is also commercially available for e.g. lactate measurements, but this enzyme has not been characterized before in detail. Here we report structural characterization of the recombinant enzyme and its co-factor dependent oligomerization. The crystal structures revealed two distinct conformations in the loop closing the active site, consistent with previous biochemical studies implicating the role of loop in catalysis. Despite the structural conservation of active site residues when compared toAerococcus viridanslactate oxidase we were not able to detect either oxidase or monooxygenase activity when L-lactate or other potential alpha hydroxyl acids were used as a substrate.Pediococcus acidilacticilactate oxidase is therefore an example of a misannotation of an FMN-dependent enzyme, which catalyzes likely a so far unknown oxidation reaction.
- Subjects :
- Glycerol
Flavin Mononucleotide
Crystallography, X-Ray
Biochemistry
Mixed Function Oxygenases
chemistry.chemical_compound
Catalytic Domain
Hydrogen peroxide
Enzyme Chemistry
chemistry.chemical_classification
0303 health sciences
Oxidase test
Multidisciplinary
Crystallography
biology
Physics
030302 biochemistry & molecular biology
Monomers
Biochemical Cofactors
food and beverages
Melting
Ketones
Condensed Matter Physics
Enzyme structure
Recombinant Proteins
Enzymes
Chemistry
Physical Sciences
Crystal Structure
Medicine
Aerococcus viridans
Phase Transitions
Research Article
Pyruvate
Science
Redox
Catalysis
03 medical and health sciences
Oxidoreductase
Solid State Physics
Lactic Acid
030304 developmental biology
Pediococcus acidilactici
Chemical Compounds
Substrate (chemistry)
Active site
Biology and Life Sciences
Proteins
biology.organism_classification
Polymer Chemistry
Enzyme
chemistry
Enzyme Structure
biology.protein
Enzymology
Protein Multimerization
Acids
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, 'PloS One ', vol: 15, pages: e0223870-1-e0223870-16 (2020), PLoS ONE, Vol 15, Iss 2, p e0223870 (2020)
- Accession number :
- edsair.doi.dedup.....b8a948767ac69d2dbf3eedd9876bbda0