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Effect of functionalized polycaprolactone on the behaviour of murine preosteoblasts
- Source :
- Biomaterials. 31:6468-6476
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- The efficiency of biomaterials used in bone repair depends greatly on their ability to interact with bone cells. Hence, we have functionalized polycaprolactone (PCL) films by peptides derived from the bone sialoprotein containing RGD sequence (pRGD), to increase their ability to interact with murine MC3T3-E1 preosteoblasts, and favour cell response to recombinant human bone morphogenetic protein-2 (rhBMP-2). RGE peptides (pRGE) were used as negative controls. The PCL films were hydrolyzed with NaOH and then carboxylic acid groups were activated to allow chemisorption of the peptides. Alkaline treatment increased the hydrophilicity of PCL films without significantly change their roughness. Peptide immobilization on PCL was checked by X-ray photoelectron spectroscopy. Hydrolyzed PCL films (Hydro PCL), which adsorbed fibronectin and vitronectin from serum after 1 h incubation, prevented the spreading of MC3T3-E1 preosteoblasts, while films bearing pRGD or pRGE did not. In contrast, MC3T3-E1 preosteoblasts attached to pRGD and incubated for 1 h in serum-free medium spread better than cells on Hydro PCL or pRGE. Only cells on pRGD had organized cytoskeleton, phosphorylated focal adhesion kinase on Y(397) and responded to rhBMP-2 by activating Smad pathway. Thus, pRGD PCL may be used to favour bone cell cytoskeletal organization and response to rhBMP-2.
- Subjects :
- Bone sialoprotein
Materials science
Polyesters
Biophysics
Bone Morphogenetic Protein 2
Biocompatible Materials
Smad Proteins
Bioengineering
macromolecular substances
Bone morphogenetic protein 2
Biomaterials
Focal adhesion
Mice
chemistry.chemical_compound
Transforming Growth Factor beta
Bone cell
Animals
Humans
Vitronectin
Cytoskeleton
Osteoblasts
biology
technology, industry, and agriculture
3T3 Cells
Vinculin
musculoskeletal system
equipment and supplies
Recombinant Proteins
Fibronectins
Fibronectin
Biochemistry
chemistry
Mechanics of Materials
Focal Adhesion Protein-Tyrosine Kinases
Bone Morphogenetic Proteins
Polycaprolactone
Wettability
Ceramics and Composites
biology.protein
Adsorption
Oligopeptides
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....b8a7b2e28df96cdbbb28479615feb0b4