lncRNA HOTAIR knockdown suppresses gastric cancer cell biological activities

Authors :: Ma Yong
Zhu Yu
Zhou Jin
Feng Yongheng
Pan Chao
Yang Kun-xing
Yang Shi-yong
Source :: Food Science & Nutrition, Food Science & Nutrition, 9(1):123-134, Food Science & Nutrition, Vol 9, Iss 1, Pp 123-134 (2021)
Publication Year :: 2020
Publisher :: John Wiley and Sons Inc., 2020.
Abstract: The aim of this study was to characterize the involvement of long noncoding RNA HOTAIR in gastric cancer development. Measurement of HOTAIR and miRNA‐206 expression by in situ hybridization (ISH) and analyzed for the correlation between HOTAIR and miRNA‐206 in gastric cancer tissues. To evaluate the effects of HOTAIR in gastric cancer, MTT assay, flow cytometry, transwell, and wound healing assays were applied. To explain the mechanism behind HOTAIR's involvement, the expression of proteins related to it was also measured by Western blotting. Finally, correlations among related factors were determined by a luciferase target experiment. HOTAIR expression significantly increased, and miRNA‐206 expression significantly decreased in cancer tissues (p<br />HOTAIR knockdown had antitumor effects by suppressing CCND1 and CCND2 expression by stimulating miRNA‐206 in gastric cancer in vitro study.

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