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IN VITRO CULTURE OF VARIOUS SPECIES OF MICROSPORIDIA CAUSING KERATITIS: EVALUATION OF THREE IMMORTALIZED CELL LINES
- Source :
- Indian Journal of Medical Microbiology, Vol 27, Iss 1, Pp 35-39 (2009)
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Being intracellular parasites, microsporidia can only be propagated in cell culture systems. This study evaluated three cell lines to determine the most suitable host-parasite In vitro system. Confluent monolayers of vero, SIRC, and HeLa cell lines, grown in 24-well tissue culture plates, were inoculated with varying concentrations (1 x 10 4 to 1 x 10 8 spores/mL) of Vittaforma corneae, Encephalitozoon hellem, Encephalitozoon cuniculi, and Encephalitozoon intestinalis spores. Growth was compared quantitatively at weekly intervals. Encephalitozoon species showed the highest amount of growth when cultured in vero cell line, while there was no significant difference in their growth in SIRC and HeLa cell lines. In comparison, V. corneae showed the highest growth in SIRC cells, followed by vero cells. The analytical sensitivity was found to be 1 x 10 4 spores/mL for vero cell line compared to 1 x 10 5 spores/mL for SIRC cell line and 1 x 10 7 spores/mL for HeLa cell line. HeLa cells also showed rapid disruption of cells, and the spores could not be easily distinguished from cell debris. This is the first report of the comparison of vero, SIRC, and HeLa for the propagation of microsporidial spores. Vero cell line was found to be more sensitive than SIRC and HeLa cells, and we believe that the inclusion of vero cell line in the routine culture protocols of ocular parasitology laboratories would result in a significant increase in the diagnostic yield.
- Subjects :
- Microbiology (medical)
lcsh:QR1-502
Colony Count, Microbial
Sensitivity and Specificity
lcsh:Microbiology
Cell Line
Microbiology
HeLa
Tissue culture
Vittaforma
Chlorocebus aethiops
Microsporidiosis
Animals
Humans
Encephalitozoon cuniculi
Keratitis
microsporidia
biology
Clinical Laboratory Techniques
fungi
Encephalitozoon
biology.organism_classification
Encephalitozoon intestinalis
Spore
Cell culture
Vero cell
Rabbits
Immortalised cell line
Subjects
Details
- ISSN :
- 02550857
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Indian Journal of Medical Microbiology
- Accession number :
- edsair.doi.dedup.....b85d46f7a1d4019093b3d5c7b65ba34e